Preliminary Phytochemical Screening and In Vitro Antioxidant Activities of Parkinsonia aculeata Linn.

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 8
• Main Authors: Sharma, Sonia, Vig, Adarsh Pal
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: Acids; Antioxidants; Antioxidants - analysis; Antioxidants - chemistry; Antioxidants - pharmacology; Biphenyl Compounds - analysis; Biphenyl Compounds - metabolism; Butanols; Environmental science; Fabaceae; Fabaceae - chemistry; Free radicals; Hexanes; Oxidation-Reduction - drug effects; Parkinsonia aculeata; Picrates - analysis; Picrates - metabolism; Plant Extracts - analysis; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Polyphenols; Potassium
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2014/756184

Butanol and hexane leaves extracts of Parkinsonia aculeata L. (Fabaceae) were assessed for its antioxidant potential by in vitro methods. Phytochemical analysis and antioxidant activity of plant extracts were studied using different in vitro assays. UPLC analysis of extracts was carried out for the identification of chemical constituents. The total phenolic contents of the butanol and hexane leaf extract were 42 mgGAE/g and 34 mgGAE/g whereas flavonoid contents of these extracts were found to be 0.044 mgRE/g and 0.005 mgRE/g, respectively. Among both extracts, butanol extract shows maximum inhibition (%) of 93.88%, 80.02%, 52.06%, 94.68%, and 69.37% in DPPH, non-site-specific and site-specific, FTC, and TBA assays and absorbance of 0.852 and 0.522 in reducing power and CUPRAC assay at the highest concentration tested. The FRAP and TAC values of butanol extract were found to be 678 μM Fe(II)/g and 36 mgAAE/100 mg. UPLC analysis of extracts revealed the presence of various polyphenols. The tested plant extracts were found to possess potent antioxidant and free radical scavenging activity which may be due to the presence of flavonoids and polyphenols.