Engineering Components of the Lactobacillus S-Layer for Biotherapeutic Applications
Lactic acid bacteria (LAB) are frequently harnessed for the delivery of biomolecules to mucosal tissues. Several species of are commonly employed for this task, of which a subset are known to possess surface-layers (S-layers). S-layers are two-dimensional crystalline arrays of repeating proteinaceou...
Saved in:
Published in | Frontiers in microbiology Vol. 9; p. 2264 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
02.10.2018
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Lactic acid bacteria (LAB) are frequently harnessed for the delivery of biomolecules to mucosal tissues. Several species of
are commonly employed for this task, of which a subset are known to possess surface-layers (S-layers). S-layers are two-dimensional crystalline arrays of repeating proteinaceous subunits that form the outermost coating of many prokaryotic cell envelopes. Their periodicity and abundance have made them a target for numerous biotechnological applications. In the following review, we examine the multi-faceted S-layer protein (Slp), and its use in both heterologous protein expression systems and mucosal vaccine delivery frameworks, through its diverse genetic components: the strong native promoter, capable of synthesizing as many as 500 Slp subunits per second; the signal peptide that stimulates robust secretion of recombinant proteins; and the structural domains, which can be harnessed for both cell surface display of foreign peptides or adhesion enhancement of a host bacterium. Although numerous studies have established vaccine platforms based on one or more components of the
S-layer, this area of research still remains largely in its infancy, thus this review is meant to not only highlight past works, but also advocate for the future usage of Slps in biotherapeutic research. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 Reviewed by: Akinobu Kajikawa, Tokyo University of Agriculture, Japan; Olivia Cano Garrido, Centre for Research in Agricultural Genomics (CRAG), Spain This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology Edited by: Aleš Berlec, Jožef Stefan Institute, Slovenia |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2018.02264 |