Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium

In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using ep...

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Published inStem cell research Vol. 46; p. 101806
Main Authors Sawada, Tomoyo, Benjamin, Kynon J.M., Brandtjen, Anna C., Tietze, Ethan, Allen, Samuel J., Paquola, Apuã C.M., Kleinman, Joel E., Hyde, Thomas M., Erwin, Jennifer A.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.07.2020
Elsevier
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Summary:In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.
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ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2020.101806