3D printed bioceramic for phage therapy against bone nosocomial infections

• Published in: Materials Science & Engineering C Vol. 111; p. 110840
• Main Authors: Bouchart, Franck, Vidal, Olivier, Lacroix, Jean-Marie, Spriet, Corentin, Chamary, Shaan, Brutel, Annie, Hornez, Jean-Christophe
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2020; Elsevier BV; Elsevier
• Subjects: Animals; Anti-infectious; Antibiotics; Bacteriophages - drug effects; Bioceramics; Biofilm; Biofilms; Biofilms - drug effects; Biomaterials; Biomedical materials; Bone surgery; Calcium phosphates; Calcium Phosphates - pharmacology; Cell Line; Cell Proliferation - drug effects; Ceramics; Ceramics - therapeutic use; Chemical Sciences; Colonization; Cross Infection - microbiology; Cross Infection - therapy; Cytoprotection - drug effects; Escherichia coli - drug effects; Escherichia coli - growth & development; Escherichia coli - physiology; Escherichia coli - ultrastructure; Joints (anatomy); Life Sciences; Lithography; Materials science; Mechanical loading; Mice; Nosocomial infection; Nosocomial infections; Osteoblasts; Osteoblasts - cytology; Osteoblasts - drug effects; Phage Therapy; Phages; Plankton - drug effects; Printing, Three-Dimensional; Proliferation; Prostheses; Side effects; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Staphylococcus aureus - physiology; Staphylococcus aureus - ultrastructure; Surface Properties; Surgery; Therapy; Three dimensional printing; X-Ray Diffraction; β-Tricalcium phosphate; Biofilm; Proliferation; Anti-infectious; Phage therapy; β-Tricalcium phosphate; Biomaterials
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.110840

This study provides a new therapeutic response to postoperative joint and bone infections. Alone or in combination with antibiotics, phage therapy has many advantages, including accurate targeting of pathogenic bacteria. In addition, a decrease in harmful side effects can improve the healing process. Integrating the bacteriophage directly into the graft product will improve the antibacterial spread over the site of the surgery. The phage cocktail-filled ceramics are an innovative device for localized and curative phage therapy (in prosthetic replacement surgery, for example) in bone and joint surgery. Calcium phosphate-based ceramics were synthesized and shaped by stereolithography (3D) before loading by a phage cocktail to lyse a heterospecific bacterial population. In addition, the device makes possible the protection of osteoblastic cells against Staphylococcus aureus infection during their colonization on the ceramic material and prevents the formation of biofilm on the surface of biomaterials. •The ß-TCP material loaded with phage cocktail have an antibacterial activity on heterospecific bacterial population.•The ß-TCP materials loaded phage makes possible the protection of osteoblastic cells against S. aureus infection.•The ß-TCP materials loaded phage prevents the formation of biofilm on the surface of biomaterials•The ß-TCP materials loaded Remus phage destroy a 24h matured S. aureus biofilm