An in situ-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce

• Published in: Frontiers in microbiology Vol. 10; p. 3089
• Main Authors: Sarengaowa, Hu, Wenzhong, Feng, Ke, Jiang, Aili, Xiu, Zhilong, Lao, Ying, Li, Yuanzheng, Long, Ya
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 05.02.2020
• Subjects: Escherichia coli O157:H7; in situ-synthesized gene chip; Listeria monocytogenes; Microbiology; Salmonella Typhimurium; Staphylococcus aureus; Vibrio parahemolyticus; tiling array; Vibrio parahemolyticus; fresh-cut fruits and vegetables; Salmonella Typhimurium; Listeria monocytogenes; Escherichia coli O157:H7; Staphylococcus aureus; in situ-synthesized gene chip
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.03089

Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people's life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An -synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific sequences of Typhimurium, , , , and O157:H7 from the National Center for Biotechnology Information database. Tiling array probes were designed to target selected genes in an optimized hybridization system. A total of 141 specific probes were selected from 3,227 hybridization probes, comprising 26 , 24 , 25 O157:H7, 20 Typhimurium, and 46 probes that are unique to this study. The optimized assay had strong amplification signals and high accuracy. The detection limit for the five target pathogens on fresh-cut cantaloupe and lettuce was approximately 3 log cfu/g without culturing and with a detection time of 24 h. The detection technology established in this study can rapidly detect and monitor the foodborne pathogens on fresh-cut fruits and vegetables throughout the logistical distribution chain, i.e., processing, cleaning, fresh-cutting, packaging, storage, transport, and sale, and represents a valuable technology that support the safety of fresh agricultural products.