Extremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm

is an opportunistic pathogen that causes high morbidity and mortality rates due to its biofilm form. Biofilm formation is regulated via quorum sensing (QS) mechanism and provides up to 1000 times more resistance against conventional antibiotics. QS related genes are expressed according to bacterial...

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Published inFrontiers in microbiology Vol. 11; p. 79
Main Authors Başaran, Tunahan Irmak, Berber, Didem, Gökalsın, Barış, Tramice, Annabella, Tommonaro, Giuseppina, Abbamondi, Gennaro Roberto, Erginer Hasköylü, Merve, Toksoy Öner, Ebru, Iodice, Carmine, Sesal, Nüzhet Cenk
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 06.02.2020
Subjects
antivirulence
biofilm
extremophiles
Microbiology
Natrinema versiforme
Pseudomonas aeruginosa
quorum sensing
Natrinema versiforme
Pseudomonas aeruginosa
quorum sensing
extremophiles
biofilm
antivirulence
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Summary:is an opportunistic pathogen that causes high morbidity and mortality rates due to its biofilm form. Biofilm formation is regulated via quorum sensing (QS) mechanism and provides up to 1000 times more resistance against conventional antibiotics. QS related genes are expressed according to bacterial population density via signal molecules. QS inhibitors (QSIs) from natural sources are widely studied evaluating various extracts from extreme environments. It is suggested that extremely halophilic Archaea may also produce QSI compounds. For this purpose, we tested QS inhibitory potentials of ethyl acetate extracts from cell free supernatants and cells of against QS and biofilm formation of . To observe QS inhibition, all extracts were tested on , , and biosensor strains and biofilm inhibition was studied using PAO1. According to our results, QS inhibition ratios of cell free supernatant extract (CFSE) were higher than cell extract (CE) on system, whereas CE was more effective on system. In addition, anti-biofilm effect of CFSE was higher than CE. Structural analysis revealed that the most abundant compound in the extracts was 4-(2-carboxy-vinyl) benzoic acid.
Bibliography:Edited by: Vincenzo De Feo, University of Salerno, Italy
Reviewed by: Rodolfo García-Contreras, National Autonomous University of Mexico, Mexico; Zaixiang Lou, Jiangnan University, China
This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.00079