Switchable Nitroproteome States of Phytophthora infestans Biology and Pathobiology

• Published in: Frontiers in microbiology Vol. 10; p. 1516
• Main Authors: Izbiańska, Karolina, Floryszak-Wieczorek, Jolanta, Gajewska, Joanna, Gzyl, Jarosław, Jelonek, Tomasz, Arasimowicz-Jelonek, Magdalena
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 16.07.2019
• Subjects: Microbiology; pathogen offensive strategy; peroxynitrite; Phytophthora infestans; potato; protein tyrosine nitration; reactive nitrogen species; late blight; peroxynitrite; potato; Phytophthora infestans; pathogen offensive strategy; reactive nitrogen species; protein tyrosine nitration
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.01516

The study demonstrates protein tyrosine nitration as a functional post-translational modification (PTM) in biology and pathobiology of the oomycete (Mont.) de Bary, the most harmful pathogen of potato ( L.). Using two isolates differing in their virulence toward potato cv. Sarpo Mira we found that the pathogen generates reactive nitrogen species (RNS) in hyphae and mature sporangia growing under and conditions. However, acceleration of peroxynitrite formation and elevation of the nitrated protein pool within pathogen structures were observed mainly during the MP 946-potato interaction. Importantly, the nitroproteome profiles varied for the pathogen virulence pattern and comparative analysis revealed that MP 977 represented a much more diverse quality spectrum of nitrated proteins. Abundance profiles of nitrated proteins that were up- or downregulated were substantially different also between the analyzed growth phases. Briefly, growth of and was accompanied by exclusive nitration of proteins involved in energy metabolism, signal transduction and pathogenesis. Importantly, the potato interaction indicated cytosolic RXLRs and Crinklers effectors as potential sensors of RNS. Taken together, we explored the first plant pathogen nitroproteome. The results present new insights into RNS metabolism in indicating protein nitration as an integral part of pathogen biology, dynamically modified during its offensive strategy. Thus, the nitroproteome should be considered as a flexible element of the oomycete developmental and adaptive mechanism to different micro-environments, including host cells.