Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae
Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of . A mutant strain (Δ ) lacking Lip40 and a complement...
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| Published in | Frontiers in microbiology Vol. 9; p. 1472 |
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| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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03.07.2018
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| Abstract | Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of
. A mutant strain (Δ
) lacking Lip40 and a complemented strain (CΔ
) were constructed. Δ
exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δ
mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δ
showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of
. |
|---|---|
| AbstractList | Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of
. A mutant strain (Δ
) lacking Lip40 and a complemented strain (CΔ
) were constructed. Δ
exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δ
mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δ
showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of
. Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of Actinobacillus pleuropneumoniae . A mutant strain (Δ lip40 ) lacking Lip40 and a complemented strain (CΔ lip40 ) were constructed. Δ lip40 exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δ lip40 mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δ lip40 showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of A. pleuropneumoniae . Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of Actinobacillus pleuropneumoniae. A mutant strain (Δlip40) lacking Lip40 and a complemented strain (CΔlip40) were constructed. Δlip40 exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δlip40 mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δlip40 showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of A. pleuropneumoniae. |
| Author | Gao, Qishuang Cao, Yurou Zhao, Jin Liu, Jinlin Yang, Dengfu Meng, Jianzhong Gao, Lulu Zhang, Li Gong, Siying Qi, Chao Zhao, Haobin Yang, Jihong |
| AuthorAffiliation | 3 Department of Animal Biotechnology and Cell Engineering, Wuhan Institute of Animal Husbandry and Veterinary Sciences, Wuhan Academy of Agricultural Sciences , Wuhan , China 1 Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University , Wuhan , China 2 Lichuan Municipal Bureau of Animal Husbandry and Veterinary Medicine , Lichuan , China |
| AuthorAffiliation_xml | – name: 1 Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University , Wuhan , China – name: 2 Lichuan Municipal Bureau of Animal Husbandry and Veterinary Medicine , Lichuan , China – name: 3 Department of Animal Biotechnology and Cell Engineering, Wuhan Institute of Animal Husbandry and Veterinary Sciences, Wuhan Academy of Agricultural Sciences , Wuhan , China |
| Author_xml | – sequence: 1 givenname: Jinlin surname: Liu fullname: Liu, Jinlin organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 2 givenname: Yurou surname: Cao fullname: Cao, Yurou organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 3 givenname: Lulu surname: Gao fullname: Gao, Lulu organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 4 givenname: Li surname: Zhang fullname: Zhang, Li organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 5 givenname: Siying surname: Gong fullname: Gong, Siying organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 6 givenname: Jihong surname: Yang fullname: Yang, Jihong organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 7 givenname: Haobin surname: Zhao fullname: Zhao, Haobin organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 8 givenname: Dengfu surname: Yang fullname: Yang, Dengfu organization: Lichuan Municipal Bureau of Animal Husbandry and Veterinary Medicine, Lichuan, China – sequence: 9 givenname: Jin surname: Zhao fullname: Zhao, Jin organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 10 givenname: Jianzhong surname: Meng fullname: Meng, Jianzhong organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China – sequence: 11 givenname: Qishuang surname: Gao fullname: Gao, Qishuang organization: Department of Animal Biotechnology and Cell Engineering, Wuhan Institute of Animal Husbandry and Veterinary Sciences, Wuhan Academy of Agricultural Sciences, Wuhan, China – sequence: 12 givenname: Chao surname: Qi fullname: Qi, Chao organization: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China |
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| Cites_doi | 10.1556/004.2015.041 10.1371/journal.pone.0001450 10.3390/ijms16022626 10.1016/S0378-1097(03)00064-8 10.1111/j.1574-6968.2007.00813.x 10.1016/S1286-4579(01)01534-9 10.1016/j.vetmic.2009.01.011 10.1002/pmic.200600979 10.1371/journal.pone.0176374 10.1016/S0034-5288(18)32364-6 10.1051/vetres/2010037 10.1016/j.ijid.2008.08.026 10.1128/IAI.00682-10 10.1016/j.micpath.2011.02.002 10.1016/j.vetmic.2015.02.026 10.1146/annurev-micro-090110-102859 10.2460/javma.1994.204.09.1334 10.1016/S0264-410X(00)00076-1 10.3389/fmicb.2017.00811 10.1128/IAI.00297-08 10.1016/0923-2508(92)90018-J 10.1146/annurev-micro-091014-104209 10.1128/JVI.00042-10 10.1099/jmm.0.000544 10.1016/j.vetmic.2015.11.021 10.1002/1873-3468.12273 10.1016/j.vetmic.2010.01.027 10.1186/s12896-015-0199-8 10.1016/S0378-1135(00)00345-X 10.1111/j.1574-6968.1999.tb08721.x 10.1128/CVI.00616-12 10.1111/j.1574-6968.2002.tb11145.x |
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| Keywords | virulence Actinobacillus pleuropneumoniae colonization adherence Lip40 |
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| Title | Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/30018613 https://search.proquest.com/docview/2071577997 https://pubmed.ncbi.nlm.nih.gov/PMC6038445 https://doaj.org/article/cd64f18cea3c4d57a50b214254d6974e |
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