Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae

Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of . A mutant strain (Δ ) lacking Lip40 and a complement...

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Published inFrontiers in microbiology Vol. 9; p. 1472
Main Authors Liu, Jinlin, Cao, Yurou, Gao, Lulu, Zhang, Li, Gong, Siying, Yang, Jihong, Zhao, Haobin, Yang, Dengfu, Zhao, Jin, Meng, Jianzhong, Gao, Qishuang, Qi, Chao
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 03.07.2018
Subjects
Actinobacillus pleuropneumoniae
adherence
colonization
Lip40
Microbiology
virulence
virulence
Actinobacillus pleuropneumoniae
colonization
adherence
Lip40
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Summary:Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of . A mutant strain (Δ ) lacking Lip40 and a complemented strain (CΔ ) were constructed. Δ exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δ mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δ showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of .
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Edited by: Yuji Morita, Meiji Pharmaceutical University, Japan
Reviewed by: Martha H. Mulks, Michigan State University, United States; Shivangi Agarwal, Northwestern University, United States
This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2018.01472