The cytochrome c gene from the green alga Chlamydomonas reinhardtii: Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
The expression of the Chlamydomonas reinhardtii cytochrome c gene was studied at the steady-state mRNA level. The inclusion of acetate under illumination produced a marked increase in cytochrome c transcripts. This effect was not affected by two inhibitors of mitochondrial energy metabolism. Three d...
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Published in | Plant and cell physiology Vol. 41; no. 10; pp. 1149 - 1156 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Japan
Oxford University Press
01.10.2000
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | The expression of the Chlamydomonas reinhardtii cytochrome c gene was studied at the steady-state mRNA level. The inclusion of acetate under illumination produced a marked increase in cytochrome c transcripts. This effect was not affected by two inhibitors of mitochondrial energy metabolism. Three different obligate photoautotrophic mutants with defective mitochondria showed normal levels of induction, suggesting that utilization of acetate for respiration is not required for this process. Light, in the presence or absence of acetate, also promoted an increase in cytochrome c transcript levels. This effect could be abolished by treatment of the cells with an inhibitor of the photosynthetic electron transport chain, suggesting that light acts through photosynthesis to promote the induction. In addition, a genomic clone encompassing the Chlamydomonas cytochrome c gene has been isolated and analyzed. The gene contains three introns, two of which are located at positions similar to those in the rice and Arabidopsis cytochrome c genes, indicating the existence of an evolutionary link. It is concluded that the cytochrome c gene from C. reinhardtii is subject to metabolic regulation through a mechanism that responds to the intracellular level of either acetate or a compound derived from its metabolization through a pathway different from mitochondrial respiration. |
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Bibliography: | F30 2001002472 istex:A490860804CE0A78DDC19470F157A1593B21F413 local:pcd044 (Recieved April 18, 2000; Accepted August 7, 2000). ark:/67375/HXZ-0KQQRKH0-X ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0032-0781 1471-9053 |
DOI: | 10.1093/pcp/pcd044 |