Transcriptome Analysis of Immune Response of mIgM + B Lymphocytes in Japanese Flounder ( Paralichthys olivaceus ) to Lactococcus lactis in vitro Revealed That IFN I-3 Could Enhance Their Phagocytosis
B cells have recently been proven to have phagocytic activities, but few studies have explored the relevant regulation mechanisms. In this study, we showed that the Japanese flounder ( ) membrane-bound (m)IgM B lymphocyte population could phagocytose inactivated with a mean phagocytic rate of 25%. H...
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Published in | Frontiers in immunology Vol. 10; p. 1622 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
16.07.2019
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Subjects | |
Online Access | Get full text |
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Summary: | B cells have recently been proven to have phagocytic activities, but few studies have explored the relevant regulation mechanisms. In this study, we showed that the Japanese flounder (
) membrane-bound (m)IgM
B lymphocyte population could phagocytose inactivated
with a mean phagocytic rate of 25%. High-purity mIgM
B lymphocytes were subsequently sorted to investigate the cellular response to
stimulation
. Transcriptome analysis identified 1,375 differentially expressed genes (DEGs) after
stimulation, including 975 upregulated and 400 downregulated genes. Many of these DEGs were enriched in multiple pathways associated with phagocytosis such as focal adhesion, the phagosome, and actin cytoskeleton regulation. Moreover, many genes involved in phagolysosomal function and antigen presentation were also upregulated after stimulation, indicating that mIgM
B lymphocytes may degrade the internalized bacteria and present processed antigenic peptides to other immune cells. Interestingly, the type I interferon 3 (
gene was upregulated after
stimulation, and further analysis showed that the recombinant (r)IFN I-3 significantly enhanced phagocytosis of
and
by mIgM
B lymphocytes. In addition, significantly higher intracellular reactive oxygen species (ROS) levels were detected in mIgM
B lymphocytes following rIFN I-3 treatment. We also found that IFN I-3 significantly upregulated
expression in mIgM
B lymphocytes, and the enhancing effect of IFN I-3 on mIgM
B lymphocyte-mediated phagocytosis was suppressed by fludarabine treatment. Collectively, these results demonstrate that mIgM
B cell-mediated phagocytosis in the Japanese flounder is effectively triggered by bacterial stimulation, and further enhanced by IFN I-3, which itself may be regulated by Stat1. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Comparative Immunology, a section of the journal Frontiers in Immunology Edited by: Andrew C. Barnes, University of Queensland, Australia Reviewed by: Uwe Fischer, Friedrich Loeffler Institute, Germany; Natalie Christina Steinel, University of Massachusetts Lowell, United States; Ikuo Hirono, Tokyo University of Marine Science and Technology, Japan |
ISSN: | 1664-3224 1664-3224 |
DOI: | 10.3389/fimmu.2019.01622 |