Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia

• Published in: Parasitology research (1987) Vol. 104; no. 2; pp. 475 - 479
• Main Authors: Kim, Juri, Bae, Sung-Su, Sung, Moon-Hee, Lee, Kyu-Ho, Park, Soon-Jung
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.01.2009; Springer-Verlag; Springer
• Subjects: Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cysts - chemistry; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; General aspects; General aspects and techniques. Study of several systematic groups. Models; Giardia lamblia - chemistry; Immunology; Invertebrates; Medical Microbiology; Microbiology; Polymerase Chain Reaction - methods; Proteome - analysis; Protozoan Proteins - analysis; Short Communication; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trophozoites - chemistry; Ornithine Carbamoyltransferase; Protein Spot; Comparative Proteomic Analysis; Vacuolar ATPase; Bovine Bile; Parasite; Protozoa; Giardia lamblia; Diplomonadida; Trophozoite; Cyst
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-008-1223-x

The proteome of Giardia lamblia at its cyst stage was compared with that of trophozoites by using two-dimensional SDS-PAGE gel electrophoresis. Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeletal proteins, metabolic enzymes, a cell-cycle-specific kinase, stress resistance proteins, and a protein involved in translation. Expression patterns of five of the identified proteins were examined during encystation by real-time PCR. Expression of cwp1 (encoding cyst wall protein 1), a marker for encystation, was increased 11-fold. In contrast, tim (encoding triose-1-phosphate isomerase) was expressed constitutively during encystation, and its transcription level was therefore used as a mRNA loading control. Expression of three genes encoding β-tubulin, vacuolar ATPase, and never-in-mitosis-A-related protein kinase did not vary significantly during encystation. Interestingly, genes encoding two heat shock proteins (Hsp70 and Hsp90) showed increased expression during encystation, suggesting that this differentiation process accompanies a cellular response to stress in G. lamblia.