Micro-Evolution Analysis Reveals Diverged Patterns of Polyol Transporters in Seven Gramineae Crops

• Published in: Frontiers in genetics Vol. 11; p. 565
• Main Authors: Kong, Weilong, Sun, Tong, Zhang, Chenhao, Qiang, Yalin, Li, Yangsheng
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 19.06.2020
• Subjects: duplicate gene pairs; functional differentiation; Genetics; Gramineae crops; orthogroups; orthologous gene pairs; polyol transporters; orthologous gene pairs; polyol transporters; functional differentiation; Gramineae crops; orthogroups; duplicate gene pairs
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2020.00565

Polyol transporters (PLTs), also called polyol/monosaccharide transporters, is of significance in determining plant development and sugar transportation. However, the diverged evolutionary patterns of the gene family in Gramineae crops are still unclear. Here a micro-evolution analysis was performed among the seven Gramineae representative crops using whole-genome sequences, i.e., (Bd), (Hv), (Or), (Os), (Sb), (Si), and (Zm), leading to the identification of 12, 11, 12, 15, 20, 24, and 20 genes, respectively. In this study, all genes were divided into nine orthogroups (OGs). However, the number of genes and the distribution of OGs were not the same in these seven Gramineae species, and different OGs were also subject to different purification selection pressures. These results indicated that the OGs of the gene family have been expanded or lost unevenly in all tested species. Then, our results of gene duplication events confirmed that gene duplication events promoted the expansion of the gene family in some Gramineous plants, namely, Bd, Or, Os, Si, Sb, and Zm, but the degree of gene family expansion, the type of gene duplication, and the differentiation time of duplicate gene pairs varied greatly among these species. In addition, the sequence alignment and the internal repeat analysis of all PLTs protein sequences implied that the PLT protein sequences may originate from an internal repeat duplication of an ancestral six transmembrane helical units. Besides that, the protein motifs result highlighted that the PLT protein sequences were highly conserved, whereas the functional differentiation of the genes was characterized by different gene structures, upstream elements, as well as co-expression analysis. The gene expression analysis of rice and maize showed that the genes have a wide range of expression patterns, suggesting diverse biological functions. Taken together, our finding provided a perspective on the evolution differences and the functional characterizations of genes in Gramineae representative crops.