Marinobacterium coralli sp. nov., isolated from mucus of coral (Mussismilia hispida)

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 1; pp. 60 - 64
• Main Authors: CHIMETTO, Luciane A, CLEENWERCK, Ilse, BROCCHI, Marcelo, WILLEMS, Anne, DE VOS, Paul, THOMPSON, Fabiano L
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 2011
• Subjects: Alteromonadaceae - classification; Alteromonadaceae - genetics; Alteromonadaceae - isolation & purification; Alteromonadaceae - physiology; Animals; Anthozoa - microbiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Brazil; Catalase - metabolism; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Marinobacterium; Microbiology; Molecular Sequence Data; Mussismilia hispida; Nucleic Acid Hybridization; Oxidoreductases - metabolism; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sodium Chloride - metabolism; Systematics; Temperature; Brazil; ASW, Brazil, Sao Paulo, Sao Sebastiao Channel; ASW, Brazil, Sao Paulo; ASW, Brazil; Coelenterata; Coral; Taxonomy; Cnidaria; Bacteria; New species; Mucus; Invertebrata
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijs.0.021105-0

A Gram-negative, aerobic bacterium, designated R-40509(T), was isolated from mucus of the reef builder coral (Mussismilia hispida) located in the São Sebastião Channel, São Paulo, Brazil. The strain was oxidase-positive and catalase-negative, and required Na(+) for growth. Its phylogenetic position was in the genus Marinobacterium and the closest related species were Marinobacterium sediminicola, Marinobacterium maritimum and Marinobacterium stanieri; the isolate exhibited 16S rRNA gene sequence similarities of 97.5-98.0 % with the type strains of these species. 16S rRNA gene sequence similarities with other type strains of the genus Marinobacterium were below 96 %. DNA-DNA hybridizations between strain R-40509(T) and the type strains of the phylogenetically closest species of the genus Marinobacterium revealed less than 70 % DNA-DNA relatedness, supporting the novel species status of the strain. Phenotypic characterization revealed that the strain was able to grow at 15-42 °C and in medium containing up to 9 % NaCl. The isolate could be differentiated from phenotypically related species by several features, including its ability to utilize d-alanine, l-alanine, bromosuccinic acid, β-hydroxybutyric acid and α-ketovaleric acid, but not acetate or l-arabinose. It produced acetoin (Voges-Proskauer), but did not have esterase lipase (C8) or catalase activities. It possessed C(18 : 1)ω7c (35 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 25 %) and C(16 : 0) (22 %) as major cellular fatty acids. The DNA G+C content was 58.5 mol%. The name Marinobacterium coralli sp. nov. is proposed to accommodate this novel isolate; the type strain is R-40509(T) (=LMG 25435(T) =CAIM 1449(T)).