Effects of Acetone Fraction From Buchenavia tomentosa Aqueous Extract and Gallic Acid on Candida albicans Biofilms and Virulence Factors
A promising anti- activity of extracts was recently described. In the present work, experiments were carried out to determine the fraction with higher antifungal activity from a extract. Acetone fraction (AF) was obtained from the aqueous extract from dried leaves (5 min/100°C) and it was the most e...
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Published in | Frontiers in microbiology Vol. 9; p. 647 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
05.04.2018
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Subjects | |
Online Access | Get full text |
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Summary: | A promising anti-
activity of
extracts was recently described. In the present work, experiments were carried out to determine the fraction with higher antifungal activity from a
extract. Acetone fraction (AF) was obtained from the aqueous extract from dried leaves (5 min/100°C) and it was the most effective one. Gallic acid (GA) was identified by electrospray ionization mass spectrometry (ESI-MS) and also chosen to perform antifungal tests due to its promising activity on
. Minimal inhibitory and fungicidal concentrations (MIC and MFC) were determined by broth microdilution technique. The effect on virulence factors of
was evaluated, and the cytotoxicity was determined. MIC
and MIC
values were both equal to 0.625 mg ml
for AF and 2.5 and 5 mg ml
, respectively, for GA. AF and GA showed ability to inhibit
adherence and to disrupt 48 h-biofilm. AF and GA were effective in reducing the formation of hyphae of
SC5314. AF and GA decreased adherence of
to oral epithelial cells. AF and GA showed slight to moderate toxicity to Vero cells. This result suggests further studies for topic use of these compounds. AF, which contains a combination of several molecules, presented greater potential of antimicrobial activity than GA, with lower values of MIC and lower cytoxicity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Edvaldo Antonio Ribeiro Rosa, Pontifícia Universidade Católica do Paraná, Brazil Reviewed by: Taissa Vila, University of Maryland, Baltimore, United States; Sudhanshu Shukla, Amity University, Gurgaon, India This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2018.00647 |