Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties

N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were coated with...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in molecular biosciences Vol. 6; p. 63
Main Authors Gupta, Kshitiz, Chhibber, Sanjay
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 06.08.2019
Subjects
antibiofilm activity
antivirulence
cell cytotoxicity
lactonase
Molecular Biosciences
silver nanoparticles
cell cytotoxicity
silver nanoparticles
lactonase
antibiofilm activity
antivirulence
Online AccessGet full text

Cover

More Information
Summary:N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were coated with N-acylated homoserine lactonase protein (AgNPs-AiiA) isolated from sp. ZA12. The AgNPs-AiiA complex was characterized by UV-visible spectra, Dynamic light Scattering, Fourier transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscope (Fe-SEM). The synthesized nano-particles were found to be spherical in shape and had an approximate size of 22.4 nm. Treatment with AiiA coated AgNPs showed a significant reduction in exopolysaccharide production, metabolic activity, cell surface hydrophobicity of bacterial cells, and anti-biofilm activity against multidrug-resistant as compared to treatment with AiiA protein and neat AgNPs. AgNPs-AiiA complex exhibited potent antibiofilm activity at sub-optimal concentration of 14.4 μg/mL without being harmful to the macrophages and to the various tissues including kidney, liver, spleen and lungs of BALB/c mice upon intra-venous administration. It is concluded that at a concentration of 14.4 μg/mL, AgNPs coated with AiiA kill bacteria without harming the host tissue and provides a suitable template to design novel anti-biofilm drug to circumvent the issue of drug resistance.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
This article was submitted to Protein Chemistry and Enzymology, a section of the journal Frontiers in Molecular Biosciences
Reviewed by: Liangliang Zhu, Fudan University, China; Muthusamy Govarthanan, University of Seoul, South Korea
Edited by: Qi Zhang, Fudan University, China
ISSN:2296-889X
2296-889X
DOI:10.3389/fmolb.2019.00063