Infrared reflection–absorption spectroscopy: Principles and applications to lipid–protein interaction in Langmuir films
Infrared reflection–absorption spectroscopy (IRRAS) of lipid/protein monolayer films in situ at the air/water interface provides unique molecular structure and orientation information from the film constituents. The technique is thus well suited for studies of lipid/protein interaction in a physiolo...
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Published in | Biochimica et biophysica acta Vol. 1798; no. 4; pp. 788 - 800 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.04.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Infrared reflection–absorption spectroscopy (IRRAS) of lipid/protein monolayer films
in situ at the air/water interface provides unique molecular structure and orientation information from the film constituents. The technique is thus well suited for studies of lipid/protein interaction in a physiologically relevant environment. Initially, the nature of the IRRAS experiment is described and the molecular structure information that may be obtained is recapitulated. Subsequently, several types of applications, including the determination of lipid chain conformation and tilt as well as elucidation of protein secondary structure are reviewed. The current article attempts to provide the reader with an understanding of the current capabilities of IRRAS instrumentation and the type of results that have been achieved to date from IRRAS studies of lipids, proteins, and lipid/protein films of progressively increasing complexity. Finally, possible extensions of the technology are briefly considered. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0005-2736 0006-3002 1879-2642 |
DOI: | 10.1016/j.bbamem.2009.11.024 |