First Cryo-Scanning Electron Microscopy Images and X-Ray Microanalyses of Mucoromycotinian Fine Root Endophytes in Vascular Plants

Arbuscule-producing fine root endophytes (FRE) (previously incorrectly ) were recently placed within subphylum Mucoromycotina; the first report of arbuscules outside subphylum Glomeromycotina. Here, we aimed to estimate nutrient concentrations in plant and fungal structures of FRE and to test the ut...

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Published inFrontiers in microbiology Vol. 11; p. 2018
Main Authors Albornoz, Felipe E., Hayes, Patrick E., Orchard, Suzanne, Clode, Peta L., Nazeri, Nazanin K., Standish, Rachel J., Bending, Gary D., Hilton, Sally, Ryan, Megan H.
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 03.09.2020
Subjects
arbuscules
cryoSEM
Glomus tenue
Microbiology
Mucoromycotina
Planticonsortium
cryoSEM
Glomus tenue
Mucoromycotina
arbuscules
Planticonsortium
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Summary:Arbuscule-producing fine root endophytes (FRE) (previously incorrectly ) were recently placed within subphylum Mucoromycotina; the first report of arbuscules outside subphylum Glomeromycotina. Here, we aimed to estimate nutrient concentrations in plant and fungal structures of FRE and to test the utility of cryo-scanning electron microscopy (cryoSEM) for studying these fungi. We used replicated cryoSEM and X-ray microanalysis of heavily colonized roots of . Intercellular hyphae and hyphae in developed arbuscules were consistently very thin; 1.35 ± 0.03 μm and 0.99 ± 0.03 μm in diameter, respectively (mean ± SE). Several intercellular hyphae were often adjacent to each other forming "hyphal ropes." Developed arbuscules showed higher phosphorus concentrations than senesced arbuscules and non-colonized structures. Senesced arbuscules showed greatly elevated concentrations of calcium and magnesium. While uniformly thin hyphae and hyphal ropes are distinct features of FRE, the morphology of fully developed arbuscules, elevated phosphorus in fungal structures, and accumulation of calcium with loss of structural integrity in senesced arbuscules are similar to glomeromycotinian fungi. Thus, we provide evidence that FRE may respond to similar host-plant signals or that the host plant may employ a similar mechanism of association with FRE and AMF.
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Reviewed by: Sabine Dagmar Zimmermann, Délégation Languedoc Roussillon (CNRS), France; Bettina Hause, Leibniz-Institut für Pflanzenbiochemie (IPB), Germany; Catarina Cardoso, Technical University of Munich, Germany
Edited by: Pierre-Emmanuel Courty, INRA Centre Dijon Bourgogne Franche-Comté, France
This article was submitted to Microbe and Virus Interactions with Plants, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.02018