Seven diverse human embryonic stem cell-derived chondrogenic clonal embryonic progenitor cell lines display site-specific cell fates

• Published in: Regenerative medicine Vol. 8; no. 2; pp. 125 - 144
• Main Authors: Sternberg, Hal, Kidd, Jennifer, Murai, James T, Jiang, Jianjie, Rinon, Ariel, Erickson, Isaac E, Funk, Walter D, Wang, Qian, Chapman, Karen B, Vangsness Jr, C Thomas, West, Michael D
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.03.2013
• Subjects: Analysis; Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Biomarkers - metabolism; bone; Bone and Bones - drug effects; Bone and Bones - pathology; Bone marrow; cartilage; Cartilage - drug effects; Cartilage - metabolism; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Line; Cell Lineage - drug effects; Cell Lineage - genetics; Chondrocytes - metabolism; Chondrogenesis - drug effects; Chondrogenesis - genetics; clonal embryonic progenitor cell; Clone Cells; Collagen Type II - metabolism; embryonic stem cell; Embryonic Stem Cells - cytology; Embryonic Stem Cells - drug effects; Embryonic Stem Cells - metabolism; Gene Expression Profiling; Gene Expression Regulation; Humans; mesenchymal stem cell; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Oligonucleotide Array Sequence Analysis; osteoarthritis; Physiological aspects; Proteoglycans - metabolism; Rats; RNA, Messenger - genetics; RNA, Messenger - metabolism; Staining and Labeling; Stem Cell Transplantation; Stem cells; Tissue Engineering; Transforming Growth Factor beta - pharmacology
• ISSN: 1746-0751; 1746-076X
• DOI: 10.2217/rme.12.117

The transcriptomes of seven diverse clonal human embryonic progenitor cell lines with chondrogenic potential were compared with that of bone marrow-derived mesenchymal stem cells (MSCs). The cell lines 4D20.8, 7PEND24, 7SMOO32, E15, MEL2, SK11 and SM30 were compared with MSCs using immunohistochemical methods, gene expression microarrays and quantitative real-time PCR. In the undifferentiated progenitor state, each line displayed unique combinations of site-specific markers, including , , , , , , , , , and , but none of the lines expressed the MSC marker . The lines showed diverse responses when differentiated in the presence of combinations of TGF- 3, BMP2, 4, 6 and 7 and GDF5, with the lines 4D20.8, SK11, SM30 and MEL2 showing osteogenic markers in some differentiation conditions. The line 7PEND24 showed evidence of regenerating articular cartilage and, in some conditions, markers of tendon differentiation. The scalability of site-specific clonal human embryonic stem cell-derived embryonic progenitor cell lines may provide novel models for the study of differentiation and methods for preparing purified and identified cells types for use in therapy. Original submitted 16 October 2012; Revised submitted 22 November 2012; Published online 18 December 2012