Ecdysis Triggering Hormone Signaling (ETH/ETHR-A) Is Required for the Larva-Larva Ecdysis in Bactrocera dorsalis (Diptera: Tephritidae)

Insects must undergo ecdysis for successful development and growth, and the ecdysis triggering hormone (ETH), released by the Inka cells, is a master hormone in this process. In this study, we determined the sequence of the ETH precursor and receptors in an agriculturally important pest insect, the...

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Published inFrontiers in physiology Vol. 8; p. 587
Main Authors Shi, Yan, Jiang, Hong-Bo, Gui, Shun-Hua, Liu, Xiao-Qiang, Pei, Yu-Xia, Xu, Li, Smagghe, Guy, Wang, Jin-Jun
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 22.08.2017
Subjects
Bactrocera dorsalis
ecdysis
ecdysis-triggering hormone
larva development
Physiology
RNAi
ecdysis-triggering hormone
larva development
RNAi
ecdysis
Bactrocera dorsalis
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Summary:Insects must undergo ecdysis for successful development and growth, and the ecdysis triggering hormone (ETH), released by the Inka cells, is a master hormone in this process. In this study, we determined the sequence of the ETH precursor and receptors in an agriculturally important pest insect, the oriental fruit fly (Hendel). We identified two functionally distinct splice receptor isoforms: BdETH-R-A and BdETH-R-B, and when expressed in Chinese hamster ovary (CHO-WTA11) cells, they exhibited a high sensitivity to the two mature peptides BdETH1 and BdETH2. The transcript was detected in the tracheal tissue of the larvae. Inka cells were identified with immunohistochemical antibody staining against ETH1, and hybridization with specific DNA probes. Selective RNA silencing of or , but not of , caused developmental failure at ecdysis. The dsRNA-treated larvae displayed tracheal defects and could not shed the old cuticle followed by death. Our results demonstrated that BdETH, via activation of BdETH-R-A but not ETH-R-B, plays an essential role in regulating the process of larva-larva ecdysis in .
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Edited by: Arash Zibaee, University of Gilan, Iran
Reviewed by: Christian Wegener, University of Würzburg, Germany; Takashi Koyama, Instituto Gulbenkian de Ciência, Portugal
This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology
These authors have contributed equally to this work.
ISSN:1664-042X
1664-042X
DOI:10.3389/fphys.2017.00587