Mass identification of transcriptional units expressed from the Bombyx mori nucleopolyhedrovirus genome

• Published in: Journal of general virology Vol. 92; no. Pt 1; pp. 200 - 203
• Main Authors: KATSUMA, Susumu, KANG, Wonkyung, SHIN-I, Tadasu, OHISHI, Kazuko, KADOTA, Koji, KOHARA, Yuji, SHIMADA, Toru
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.01.2011
• Subjects: Animals; Baculovirus; Biological and medical sciences; Bombyx - virology; Bombyx mori NPV; Cell Line; DNA, Viral - chemistry; DNA, Viral - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Gene Expression Regulation, Viral; Gene Library; Genome, Viral; Microbiology; Miscellaneous; Molecular Sequence Data; Nuclear polyhedrosis virus; Nucleopolyhedrovirus - genetics; Nucleopolyhedrovirus - growth & development; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; RNA, Untranslated - biosynthesis; RNA, Untranslated - genetics; RNA, Viral - biosynthesis; RNA, Viral - genetics; Sequence Analysis, DNA; Virology; Virus; Nucleopolyhedrovirus; Baculoviridae; Arthropoda; Insecta; Bombycidae; Lepidoptera; Bombyx mori; Identification; Invertebrata; Genome
• ISSN: 0022-1317; 1465-2099; 1465-2099
• DOI: 10.1099/vir.0.025908-0

In order to identify the transcriptional units expressed from an entire nucleopolyhedrovirus (NPV) genome during infection, we constructed a full-length-enriched cDNA library from Bombyx mori NPV (BmNPV)-infected BmN cells. We randomly sequenced 11,520 clones from both ends to obtain a total of 4679 BmNPV-derived transcriptional units. The data revealed a number of novel transcripts, including putative non-coding RNAs, most of which are expressed from recognized baculovirus early or late promoter motifs. These findings provide new insights into the complex transcriptional regulation of an NPV genome and suggest roles for as-yet-uncharacterized transcripts.