Chryseobacterium lacus sp. nov. Isolated From the Surface Water of Two Lakes With Light-Induced Carotenoid Production

Two Gram-stain-negative, rod-shaped, gliding, catalase-positive, and facultative anaerobic strains, YLOS41 and XH07, were isolated from surface water of Yilong Lake and West Lake of Dali in Yunnan Province, respectively. Both strains were yellow-colored under light conditions and white-colored under...

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Published inFrontiers in microbiology Vol. 11; p. 251
Main Authors Zhang, Jing, Gao, Cheng, Yu, Xue-Mei, Lun, He-Yuan, Du, Zong-Jun
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 04.03.2020
Subjects
carotenoid synthesis
Chryseobacterium
genome
light
Microbiology
surface water
transcriptome
surface water
genome
carotenoid synthesis
Chryseobacterium
light
transcriptome
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Summary:Two Gram-stain-negative, rod-shaped, gliding, catalase-positive, and facultative anaerobic strains, YLOS41 and XH07, were isolated from surface water of Yilong Lake and West Lake of Dali in Yunnan Province, respectively. Both strains were yellow-colored under light conditions and white-colored under dark conditions. The results of physiological and chemotaxonomic characterization, sequencing and phylogenetic analysis, and draft genome sequence comparison demonstrated that the two strains represented a single novel species within the genus , for which the name sp. nov. is proposed. The type strain is YLOS41 (= KCTC 62352 = MCCC 1H00300 ), and the second strain is XH07 (= KCTC 62993). During the cultivation process, we found that the colony color of the two strains changed from white to yellow with illumination. The study investigated the effects of light irradiation on the strain YLOS41 . Results showed that light irradiation did not affect the growth of cells but significantly increased carotenoid synthesis, which caused the change of colony color. In-depth metabolic analysis was conducted by transcriptome. The predominant changes were found for genes involved in carotenoid synthesis as protection from light damage. Based on the genome and transcriptome, we proved that strain YLOS41 possessed a complete synthetic pathway of carotenoid and speculated that the production was zeaxanthin. This was the first report of species with light-induced carotenoid synthesis. This study enhances our present knowledge on how species isolated from surface water responds to light damage.
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Edited by: Martin G. Klotz, Washington State University, United States
Reviewed by: Iain Sutcliffe, Northumbria University, United Kingdom; Celia Hugo, University of the Free State, South Africa; Zhe-Xue Quan, Fudan University, China
This article was submitted to Aquatic Microbiology, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.00251