Physiological and Molecular Investigation of Urea Uptake Dynamics in Cucumis sativus L. Plants Fertilized With Urea-Doped Amorphous Calcium Phosphate Nanoparticles

• Published in: Frontiers in plant science Vol. 12; p. 745581
• Main Authors: Feil, Sebastian B, Rodegher, Giacomo, Gaiotti, Federica, Alzate Zuluaga, Monica Yorlady, Carmona, Francisco J, Masciocchi, Norberto, Cesco, Stefano, Pii, Youry
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 07.12.2021
• Subjects: cucumber; gene expression; hydroxyapatite; ionomics; nanofertilizers; Plant Science; urea uptake rate; ionomics; hydroxyapatite; nanofertilizers; urea uptake rate; cucumber; gene expression
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2021.745581

At present, the quest for innovative and sustainable fertilization approaches aiming to improve agricultural productivity represents one of the major challenges for research. In this context, nanoparticle-based fertilizers can indeed offer an interesting alternative with respect to traditional bulk fertilizers. Several pieces of evidence have already addressed the effectiveness of amorphous calcium phosphate-based nanoparticles as carriers for macronutrients, such as nitrogen (N), demonstrating increase in crop productivity and improvement in quality. Nevertheless, despite N being a fundamental nutrient for crop growth and productivity, very little research has been carried out to understand the physiological and molecular mechanisms underpinning N-based fertilizers supplied to plants nanocarriers. For these reasons, this study aimed to investigate the responses of L. to amorphous calcium phosphate nanoparticles doped with urea (U-ACP). Urea uptake dynamics at root level have been investigated by monitoring both the urea acquisition rates and the modulation of urea transporter , whereas growth parameters, the accumulation of N in both root and shoots, and the general ionomic profile of both tissues have been determined to assess the potentiality of U-ACP as innovative fertilizers. The slow release of urea from nanoparticles and/or their chemical composition contributed to the upregulation of the urea uptake system for a longer period (up to 24 h after treatment) as compared to plants treated with bulk urea. This prolonged activation was mirrored by a higher accumulation of N in nanoparticle-treated plants (approximately threefold increase in the shoot of NP-treated plants compared to controls), even when the concentration of urea conveyed through nanoparticles was halved. In addition, besides impacting N nutrition, U-ACP also enhanced Ca and P concentration in cucumber tissues, thus having possible effects on plant growth and yield, and on the nutritional value of agricultural products.