Olax scandens Mediated Biogenic Synthesis of Ag-Cu Nanocomposites: Potential Against Inhibition of Drug-Resistant Microbes

• Published in: Frontiers in chemistry Vol. 8; p. 103
• Main Authors: Mujeeb, Anzar Abdul, Khan, Nuha Abeer, Jamal, Fauzia, Badre Alam, Khan Farheen, Saeed, Haris, Kazmi, Shadab, Alshameri, Ansam Wadia Faid, Kashif, Mohammad, Ghazi, Irfan, Owais, Mohammad
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 28.02.2020
• Subjects: Ag-Cu NCs; anti-biofilm potential; antimicrobial potential; biogenic; Chemistry; reactive oxygen species (ROS); anti-biofilm potential; Ag-Cu NCs; reactive oxygen species (ROS); biogenic; antimicrobial potential
• ISSN: 2296-2646; 2296-2646
• DOI: 10.3389/fchem.2020.00103

In the present study, we have synthesized silver-copper nanocomposites (Ag-Cu NCs) using an leaf extract (green synthesis method) and evaluated their antimicrobial potential against less susceptible pathogens. The kinetics of Ag-Cu NCs synthesis was followed by UV-VIS and fluorescence spectroscopy. The physicochemical characterization of as-synthesized Ag-Cu NCs was executed using electron microscopy, Energy Dispersive X-Ray, Fourier Transform Infrared Spectroscopy, and a Differential Light Scattering method. As-synthesized Ag-Cu NCs induced the formation of Reactive Oxygen Species (ROS), thereby causing alteration and decrementation of cellular proteins, DNA, lipids, etc., and eventually leading to cell death, as determined by a Live/Dead assay. Next, we assessed the anti-biofilm potential of as-synthesized Ag-Cu NCs against biofilm forming bacteria. The as-synthesized Ag-Cu NCs, when compared to monometallic silver nanoparticles, exhibited significantly higher anti-microbial activity against both sensitive as well as drug resistant microbial isolates.