MicroRNAs Encoded by Bovine Leukemia Virus (BLV) Are Associated with Reduced Expression of B Cell Transcriptional Regulators in Dairy Cattle Naturally Infected with BLV
Bovine leukemia virus (BLV) is estimated to infect over 83% of dairy herds and over 40% of all dairy cows in the United States. While, BLV only causes leukemia in a small proportion of animals, research indicates that BLV+ cattle exhibit reduced milk production and longevity that is distinct from ly...
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Published in | Frontiers in veterinary science Vol. 4; p. 245 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
15.01.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Bovine leukemia virus (BLV) is estimated to infect over 83% of dairy herds and over 40% of all dairy cows in the United States. While, BLV only causes leukemia in a small proportion of animals, research indicates that BLV+ cattle exhibit reduced milk production and longevity that is distinct from lymphoma development. It is hypothesized that BLV negatively affects production by interfering with cattle immunity and increasing the risk of secondary infections. In particular, BLV+ cows demonstrate reduced circulating levels of both antigen-specific and total IgM. This study investigated possible mechanisms by which BLV could interfere with the production of IgM in naturally infected cattle. Specifically, total plasma IgM and the expression of genes
, and
in circulating IgM+ B cells were measured in 15 naturally infected BLV+ and 15 BLV- cows. In addition, BLV proviral load (PVL) (a relative measurement of BLV provirus integrated into host DNA) and the relative expression of BLV
and 5 BLV microRNAs (miRNAs) were characterized and correlated to the expression of selected endogenous genes. BLV+ cows exhibited lower total plasma IgM and lower expression of
, and
. While, BLV
and BLV miRNAs failed to correlate with
expression, both BLV
and BLV miRNAs exhibited negative associations with
and
gene expression. The results suggest a possible transcriptional pathway by which BLV interferes with IgM production in naturally infected cattle. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Specialty section: This article was submitted to Veterinary Infectious Diseases, a section of the journal Frontiers in Veterinary Science Edited by: Dirk Werling, Royal Veterinary College, United Kingdom Reviewed by: Kieran G. Meade, Teagasc, The Irish Agriculture and Food Development Authority, Ireland; Jean-Pierre Frossard, Animal and Plant Health Agency, United Kingdom |
ISSN: | 2297-1769 2297-1769 |
DOI: | 10.3389/fvets.2017.00245 |