Methyl Jasmonate and Sodium Nitroprusside Jointly Alleviate Cadmium Toxicity in Wheat ( Triticum aestivum L.) Plants by Modifying Nitrogen Metabolism, Cadmium Detoxification, and AsA-GSH Cycle

• Published in: Frontiers in plant science Vol. 12; p. 654780
• Main Authors: Kaya, Cengiz, Ugurlar, Ferhat, Ashraf, Muhammad, Noureldeen, Ahmed, Darwish, Hadeer, Ahmad, Parvaiz
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 05.08.2021
• Subjects: cadmium toxicity; inorganic nutrients; methyl jasmonate; oxidative stress; Plant Science; wheat; cadmium toxicity; inorganic nutrients; methyl jasmonate; wheat; oxidative stress
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2021.654780

The principal intent of the investigation was to examine the influence of joint application of methyl jasmonate (MeJA, 10 μM) and a nitric oxide-donor sodium nitroprusside (SNP, 100 μM) to wheat plants grown under cadmium (Cd as CdCl , 100 μM) stress. Cd stress suppressed plant growth, chlorophylls ( ), and PSII maximum efficiency ( / ), but it elevated leaf and root Cd, and contents of leaf proline, phytochelatins, malondialdehyde, and hydrogen peroxide, as well as the activity of lipoxygenase. MeJA and SNP applied jointly or singly improved the concentrations of key antioxidant biomolecules, e.g., reduced glutathione and ascorbic acid and the activities of the key oxidative defense system enzymes such as catalase, superoxide dismutase, dehydroascorbate reductase, glutathione -transferase, and glutathione reductase. Exogenously applied MeJA and SNP jointly or singly also improved nitrogen metabolism by activating the activities of glutamine synthetase, glutamate synthase, and nitrate and nitrite reductases. Compared with individual application of MeJA or SNP, the combined application of both showed better effect in terms of improving plant growth and key metabolic processes and reducing tissue Cd content, suggesting a putative interactive role of both compounds in alleviating Cd toxicity in wheat plants. The main findings are that exogenous application of methyl jasmonate and nitric oxide-donor sodium nitroprusside alleviated the cadmium (Cd)-induced adverse effects on growth of wheat plants grown under Cd by modulating key physiological processes and up-regulating enzymatic antioxidants and the ascorbic acid-glutathione cycle-related enzymes.