Pericyte-derived heme-binding protein 1 promotes angiogenesis and improves erectile function in diabetic mice
PURPOSETo comprehensively evaluate the effect on angiogenesis of heme-binding protein 1 (Hebp1) in the treatment of diabetes-induced erectile dysfunction. MATERIALS AND METHODSMouse corpus cavernosum endothelial cells and pericytes were used for in vitro study. Four groups of mice were used: control...
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Published in | Investigative and clinical urology Vol. 63; no. 4; pp. 464 - 474 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
The Korean Urological Association
01.07.2022
Korean Urological Association 대한비뇨의학회 |
Subjects | |
Online Access | Get full text |
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Summary: | PURPOSETo comprehensively evaluate the effect on angiogenesis of heme-binding protein 1 (Hebp1) in the treatment of diabetes-induced erectile dysfunction. MATERIALS AND METHODSMouse corpus cavernosum endothelial cells and pericytes were used for in vitro study. Four groups of mice were used: control nondiabetic mice and streptozotocin-induced diabetic mice receiving two intracavernous injections of phosphate-buffered saline, Hebp1 (1 µg), or Hebp1 (5 µg). The function of Hebp1 in diabetic conditions was evaluated by tube formation assay, aorta ring assay, migration assay, intracavernous pressure, immunofluorescence staining, and Western blot experiments. RESULTSWe report that Hebp1 is more highly expressed in mouse corpus cavernosum pericytes and can effectively promote endothelial cell angiogenesis under high-glucose conditions. Following exogenous administration of Hebp1 protein, we found that elevated Hebp1 levels can improve the erectile function of diabetic mice, which is achieved by reducing reactive oxygen species levels and activating the PI3K/AKT/eNOS signaling pathway. CONCLUSIONSOur findings demonstrate that Hebp1 can promote angiogenesis and improve erectile function under diabetic conditions. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 https://www.icurology.org/pdf/10.4111/icu.20220038 |
ISSN: | 2466-0493 2466-054X |
DOI: | 10.4111/icu.20220038 |