MC03g0810 , an Important Candidate Gene Controlling Black Seed Coat Color in Bitter Gourd ( Momordica spp.)
Seed coat color is one of the most intuitive phenotypes in bitter gourd ( spp.). Although the inheritance of the seed coat color has been reported, the gene responsible for it is still unknown. This study used two sets of parents, representing, respectively, the intersubspecific and intraspecific ma...
Saved in:
Published in | Frontiers in plant science Vol. 13; p. 875631 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
27.04.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Seed coat color is one of the most intuitive phenotypes in bitter gourd (
spp.). Although the inheritance of the seed coat color has been reported, the gene responsible for it is still unknown. This study used two sets of parents, representing, respectively, the intersubspecific and intraspecific materials of bitter gourd, and their respective F
and F
progenies for genetic analysis and primary mapping of the seed coat color. A large F
population comprising 2,975 seedlings from intraspecific hybridization was used to fine-map the seed coat color gene. The results inferred that a single gene, named
, controlled the seed coat color and that the black color was dominant over the yellow color. The
locus was mapped to a region with a physical length of ∼7.8 Mb and 42.7 kb on pseudochromosome 3
bulked segregant analysis with whole-genome resequencing (BSA-seq) and linkage analysis, respectively. Subsequently, the
locus was further fine-mapped to a 13.2-kb region containing only one candidate gene,
, encoding a polyphenol oxidase (PPO). Additionally, the variations of
in the 89 bitter gourd germplasms showed a complete correlation with the seed coat color. Expression and PPO activity analyses showed a positive correlation between the expression level of
and its product PPO and the seed coat color. Therefore,
was proposed as the causal gene of
. Our results provide an important reference for molecular marker-assisted breeding based on the seed coat color and uncover molecular mechanisms of the seed coat color formation in bitter gourd. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work Reviewed by: Mei Yang, Wuhan Botanical Garden (CAS), China; Massimo Iorizzo, North Carolina State University, United States This article was submitted to Plant Breeding, a section of the journal Frontiers in Plant Science Edited by: Muhammad Kashif Riaz Khan, Nuclear Institute for Agriculture and Biology, Pakistan |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.875631 |