Characterization of an Aldehyde Oxidoreductase From the Mesophilic Bacterium Aromatoleum aromaticum EbN1, a Member of a New Subfamily of Tungsten-Containing Enzymes

• Published in: Frontiers in microbiology Vol. 10; p. 71
• Main Authors: Arndt, Fabian, Schmitt, Georg, Winiarska, Agnieszka, Saft, Martin, Seubert, Andreas, Kahnt, Jörg, Heider, Johann
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 2019
• Subjects: aldehyde oxidoreductase; enzyme kinetics; Fe-S cluster; flavin; Microbiology; tungsten cofactor; Fe-S cluster; aldehyde oxidoreductase; tungsten cofactor; enzyme kinetics; flavin
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.00071

The biochemical properties of a new tungsten-containing aldehyde oxidoreductase from the mesophilic betaproteobacterium EbN1 (AOR ) are presented in this study. The enzyme was purified from phenylalanine-grown cells of an overexpressing mutant lacking the gene for an aldehyde dehydrogenase normally involved in anaerobic phenylalanine degradation. AOR catalyzes the oxidation of a broad variety of aldehydes to the respective acids with either viologen dyes or NAD as electron acceptors. In contrast to previously known AORs, AOR is a heterohexameric protein consisting of three different subunits, a large subunit containing the W-cofactor and an Fe-S cluster, a small subunit containing four Fe-S clusters, and a medium subunit containing an FAD cofactor. The presence of the expected cofactors have been confirmed by elemental analysis and spectrophotometric methods. AOR has a pH optimum of 8.0, a temperature optimum of 40°C and is completely inactive at 50°C. Compared to archaeal AORs, AOR is remarkably resistant against exposure to air, exhibiting a half-life time of 1 h as purified enzyme and being completely unaffected in cell extracts. Kinetic parameters of AOR have been obtained for the oxidation of one aliphatic and two aromatic aldehydes, resulting in about twofold higher values with benzyl viologen than with NAD as electron acceptor. Finally, we obtained evidence that AOR is also catalyzing the reverse reaction, reduction of benzoate to benzaldehyde, albeit at very low rates and under conditions strongly favoring acid reduction, e.g., low pH and using Ti(III) citrate as electron donor of very low redox potential. AOR appears to be a prototype of a new subfamily of bacterial AOR-like tungsten-enzymes, which differ from the previously known archaeal AORs mostly by their multi-subunit composition, their low sensitivity against oxygen, and the ability to use NAD as electron acceptor.