Downregulation of prolactin-releasing peptide gene expression in the hypothalamus and brainstem of diabetic rats

• Published in: Peptides (New York, N.Y. : 1980) Vol. 28; no. 8; pp. 1596 - 1604
• Main Authors: Mera, Takashi, Fujihara, Hiroaki, Saito, Jun, Kawasaki, Makoto, Hashimoto, Hirofumi, Saito, Takeshi, Shibata, Minori, Onaka, Tatsushi, Tanaka, Yoshiya, Oka, Takakazu, Tsuji, Sadatoshi, Ueta, Yoichi
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.08.2007; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Brain Stem - metabolism; Diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - metabolism; Diabetes. Impaired glucose tolerance; Dorsomedial Hypothalamic Nucleus - metabolism; Down-Regulation; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Fundamental and applied biological sciences. Psychology; Hyperglycemia; Hypothalamic Hormones - genetics; Hypothalamus - metabolism; In Situ Hybridization; Insulin; Insulin - pharmacology; Leptin; Leptin - pharmacology; Male; Medical sciences; Medulla Oblongata - metabolism; Neuropeptides - genetics; Obesity - drug therapy; Obesity - genetics; Obesity - metabolism; Prolactin-Releasing Hormone; Prolactin-releasing peptide; Rats; Rats, Wistar; Rats, Zucker; Restraint stress; Restraint, Physical - adverse effects; Restraint, Physical - physiology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Solitary Nucleus - metabolism; Vertebrates: endocrinology; PrRP; Diabetes mellitus; ACTH; PVN; s.c; CCK; i.p; NTS; Insulin; RTS; POMC; Hyperglycemia; Restraint stress; NA; VLM; NPY; Prolactin-releasing peptide; Leptin; i.c.v; STZ; CRH; HPA; AgRP; DMH; Endocrinopathy; Pancreatic hormone; Peptides; Rat; Brain stem; Rodentia; Central nervous system; Adipokine; Hypothalamus; Gene expression; Encephalon; Vertebrata; Mammalia; Adenohypophyseal hormone; Prolactin; Animal; Prolactin-releasing peptide,Diabetes mellitus,Hyperglycemia,Insulin,Leptin,Restraint stress
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2007.06.023

We investigated the prolactin-releasing peptide (PrRP) mRNA levels in the hypothalamus and brainstem of streptozotocin (STZ)-induced diabetic rats and fa/ fa Zucker diabetic rats, using in situ hybridization histochemistry. PrRP mRNA levels in the hypothalamus and brainstem of STZ-induced diabetic rats were significantly reduced in comparison with those of control rats. PrRP mRNA levels in the diabetic rats were reversed by both insulin and leptin. PrRP mRNA levels in the fa/ fa diabetic rats were significantly reduced in comparison with those of Fa/ ? rats. PrRP mRNA levels in the fa/ fa diabetic rats were significantly increased by insulin-treatment, but did not reach control levels in the Fa/ ? rats. We also investigated the effect of restraint stress on PrRP mRNA levels in STZ-induced diabetic rats. The PrRP mRNA levels in the control and the STZ-induced diabetic rats increased significantly after restraint stress. The diabetic condition and insulin-treatment may affect the regulation of PrRP gene expression via leptin and other factors, such as plasma glucose level. The diabetic condition may not impair the role of PrRP as a stress mediator.