Generation of a B2M homozygous knockout human somatic cell nuclear transfer-derived embryonic stem cell line using the CRISPR/Cas9 system

Beta2-microglobulin (B2M) is a subunit of human leukocyte antigen class-I (HLA-I) heterodimer that mediates immune rejection through activation of cytotoxic T cells. B2M binding to HLA-I proteins is essential for functional HLA-I on the cell surface. Here, we generated a B2M homozygous knockout soma...

Full description

Saved in:
Bibliographic Details
Published inStem cell research Vol. 59; p. 102643
Main Authors Lee, Ok-Hee, Lee, Siyoung, Park, Miseon, Moon, Sohyeon, Hwang, Semi, Kim, Byeongseok, Kim, C-Yoon, Lee, Dong Ryul, Shim, Sung Han, Park, Keun-Hong, Chung, Hyung Min, Choi, Youngsok
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.03.2022
Elsevier
Online AccessGet full text

Cover

More Information
Summary:Beta2-microglobulin (B2M) is a subunit of human leukocyte antigen class-I (HLA-I) heterodimer that mediates immune rejection through activation of cytotoxic T cells. B2M binding to HLA-I proteins is essential for functional HLA-I on the cell surface. Here, we generated a B2M homozygous knockout somatic cell nuclear transfer-induced embryonic stem cell (SCNT-ESC) line using CRISPR/Cas9-mediated gene targeting. B2M KO cell line, which does not express HLA-I molecules on cell surface, has pluripotency and differentiation ability to three germ layers. This cell line provides a useful cell source for investigating immunogenicity of allogeneic ESCs and their derivatives for tissue regeneration.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2021.102643