A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of this Field

Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein-protein i...

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Published inProteomes Vol. 8; no. 3; p. 14
Main Authors Dupree, Emmalyn J, Jayathirtha, Madhuri, Yorkey, Hannah, Mihasan, Marius, Petre, Brindusa Alina, Darie, Costel C
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 06.07.2020
MDPI AG
Subjects
mass spectrometry
protein characterization
protein identification
proteomics
Review
mass spectrometry
protein identification
proteomics
protein characterization
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Summary:Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein-protein interactions. This can be especially beneficial in the clinical setting when studying proteins involved in different diseases and conditions. Here, we aim to describe a bottom-up proteomics workflow from sample preparation to data analysis, including all of its benefits and pitfalls. We also describe potential improvements in this type of proteomics workflow for the future.
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These authors contributed equally to this work.
ISSN:2227-7382
2227-7382
DOI:10.3390/proteomes8030014