The timing of transcranial magnetic stimulation relative to the phase of prefrontal alpha EEG modulates downstream target engagement

• Published in: Brain stimulation Vol. 16; no. 3; pp. 830 - 839
• Main Authors: Pantazatos, Spiro P., Mclntosh, James R., Saber, Golbarg T., Sun, Xiaoxiao, Doose, Jayce, Faller, Josef, Lin, Yida, Teves, Joshua B., Blankenship, Aidan, Huffman, Sarah, Goldman, Robin I., George, Mark S., Sajda, Paul, Brown, Truman R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2023; Elsevier
• Subjects: DCM; Dynamic causal modeling; Electroencephalogram; Neurostimulation; Target engagement; Transcranial magnetic stimulation; Neurostimulation; Target engagement; Transcranial magnetic stimulation; Dynamic causal modeling; Electroencephalogram; DCM
• ISSN: 1935-861X; 1876-4754
• DOI: 10.1016/j.brs.2023.05.007

The communication through coherence model posits that brain rhythms are synchronized across different frequency bands and that effective connectivity strength between interacting regions depends on their phase relation. Evidence to support the model comes mostly from electrophysiological recordings in animals while evidence from human data is limited. Here, an fMRI-EEG-TMS (fET) instrument capable of acquiring simultaneous fMRI and EEG during noninvasive single pulse TMS applied to dorsolateral prefrontal cortex (DLPFC) was used to test whether prefrontal EEG alpha phase moderates TMS-evoked top-down influences on subgenual, rostral and dorsal anterior cingulate cortex (ACC). Six runs (276 total trials) were acquired in each participant. Phase at each TMS pulse was determined post-hoc using single-trial sorting. Results were examined in two independent datasets: healthy volunteers (HV) (n = 11) and patients with major depressive disorder (MDD) (n = 17) collected as part of an ongoing clinical trial. In both groups, TMS-evoked functional connectivity between DLPFC and subgenual ACC (sgACC) depended on the EEG alpha phase. TMS-evoked DLPFC to sgACC fMRI-derived effective connectivity (EC) was modulated by EEG alpha phase in healthy volunteers, but not in the MDD patients. Top-down EC was inhibitory for TMS pulses during the upward slope of the alpha wave relative to TMS timed to the downward slope of the alpha wave. Prefrontal EEG alpha phase dependent effects on TMS-evoked fMRI BOLD activation of the rostral anterior cingulate cortex were detected in the MDD patient group, but not in the healthy volunteer group. Results demonstrate that TMS-evoked top-down influences vary as a function of the prefrontal alpha rhythm, and suggest potential clinical applications whereby TMS is synchronized to the brain's internal rhythms in order to more efficiently engage deep therapeutic targets. •TMS was delivered to dorsolateral prefrontal cortex (DLPFC) at different phase points in the cycle of prefrontal alpha.•Timing of single-pulse TMS relative to prefrontal alpha was determined during simultaneous fMRI, EEG and TMS acquisition.•DLPFC-subgenual anterior cingulate cortex (sgACC) functional connectivity depends on TMS timing relative to prefrontal alpha.•Dynamic causal modeling suggests TMS delivered during the rising (falling) phase of EEG alpha inhibits (excites) the sgACC.•TMS during the trough of EEG alpha led to higher BOLD activity in the rostral ACC.