Adipose tissue infiltration in normal-weight subjects and its impact on metabolic function

• Published in: Translational research : the journal of laboratory and clinical medicine Vol. 172; pp. 6 - 17.e3
• Main Authors: Moreno-Indias, Isabel, Oliva-Olivera, Wilfredo, Omiste, Antonio, Castellano-Castillo, Daniel, Lhamyani, Said, Camargo, Antonio, Tinahones, Francisco J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2016
• Subjects: Adipose Tissue - metabolism; Anthropometry; Biomarkers - metabolism; Body Weight; Cytokines - metabolism; Female; Gene Expression Regulation; Humans; Inflammation Mediators - metabolism; Internal Medicine; Intra-Abdominal Fat - metabolism; Lipogenesis - genetics; Macrophages - metabolism; Male; Metabolic Syndrome - genetics; Metabolic Syndrome - metabolism; Middle Aged; Monocytes - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Subcutaneous Fat - metabolism; MU; VAT; MH; MHO; ATMc; SAT; metabolically unhealthy; adipose tissue monocytes/macrophages; metabolically healthy; subcutaneous adipose tissue; metabolically healthy obese; visceral adipose tissue
• ISSN: 1931-5244; 1878-1810; 1878-1810
• DOI: 10.1016/j.trsl.2016.01.002

Discordant phenotypes, metabolically healthy obese and unhealthy normal-weight individuals, are always interesting to provide important insights into the mechanistic link between adipose tissue dysfunction and associated metabolic alterations. Macrophages can release factors that impair the proper activity of the adipose tissue. Thus, studying subcutaneous and visceral adipose tissues, we investigated for the first time the differences in monocyte/macrophage infiltration, inflammation, and adipogenesis of normal-weight subjects who differed in their degree of metabolic syndrome. The study included 92 normal-weight subjects who differed in their degree of metabolic syndrome. Their anthropometric and biochemical parameters were measured. RNA from subcutaneous and visceral adipose tissues was isolated, and mRNA expression of monocyte/macrophage infiltration (CD68, CD33, ITGAM, CD163, EMR-1, CD206, MerTK, CD64, ITGAX), inflammation (IL-6, tumor necrosis factor alpha [TNFα], IL-10, IL-1b, CCL2, CCL3), and adipogenic and lipogenic capacity markers (PPARgamma, FABP4) were measured. Taken together, our data provide evidence of a different degree of macrophage infiltration between the adipose tissues, with a higher monocyte/macrophage infiltration in subcutaneous adipose tissue in metabolically unhealthy normal-weight subjects, whereas visceral adipose tissue remained almost unaffected. An increased macrophage infiltration of adipose tissue and its consequences, such as a decrease in adipogenesis function, may explain why both the obese and normal-weight subjects can develop metabolic diseases or remain healthy.