The Ankyrin-Repeat Gene GmANK114 Confers Drought and Salt Tolerance in Arabidopsis and Soybean

Ankyrin repeat (ANK) proteins are essential in cell growth, development, and response to hormones and environmental stresses. In the present study, 226 genes were identified and classified into nine subfamilies according to conserved domains in the soybean genome ( L.). Among them, the was highly in...

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Published inFrontiers in plant science Vol. 11; p. 584167
Main Authors Zhao, Juan-Ying, Lu, Zhi-Wei, Sun, Yue, Fang, Zheng-Wu, Chen, Jun, Zhou, Yong-Bin, Chen, Ming, Ma, You-Zhi, Xu, Zhao-Shi, Min, Dong-Hong
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 29.10.2020
Subjects
ankyrin repeat protein
drought and salt tolerance
genome-wide analysis
Plant Science
responsive mechanism
soybean
soybean
ankyrin repeat protein
drought and salt tolerance
genome-wide analysis
responsive mechanism
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Summary:Ankyrin repeat (ANK) proteins are essential in cell growth, development, and response to hormones and environmental stresses. In the present study, 226 genes were identified and classified into nine subfamilies according to conserved domains in the soybean genome ( L.). Among them, the was highly induced by drought, salt, and abscisic acid. The encodes a protein that belongs to the ANK-RF subfamily containing a RING finger (RF) domain in addition to the ankyrin repeats. Heterologous overexpression of in transgenic improved the germination rate under drought and salt treatments compared to wild-type. Homologous overexpression of improved the survival rate under drought and salt stresses in transgenic soybean hairy roots. In response to drought or salt stress, overexpression in soybean hairy root showed higher proline and lower malondialdehyde contents, and lower H O and O contents compared control plants. Besides, activated transcription of several abiotic stress-related genes, including , , , , and under drought and salt stresses in soybean. These results provide new insights for functional analysis of soybean ANK proteins and will be helpful for further understanding how ANK proteins in plants adapt to abiotic stress.
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Edited by: Zhi Chang Chen, Fujian Agriculture and Forestry University, China
Reviewed by: Dezhi Wu, Zhejiang University, China; Umesh K. Reddy, West Virginia State University, United States
These authors have contributed equally to this work
Present address: Zhi-Wei Lu, Zhanjiang City Key Laboratory for Tropical Crops Genetic Improvement, South Subtropical Crops Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China
This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2020.584167