Eugenol Induces Phenotypic Alterations and Increases the Oxidative Burst in Cryptococcus
Eugenol is a phenolic compound and the main constituent of the essential oil of clove India. Although there are reports of some pharmacological effects of eugenol, this study is the first that proposes to evaluate the antifungal effects of this phenol against both and cells. The effect of eugenol ag...
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Published in | Frontiers in microbiology Vol. 8; p. 2419 |
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Main Authors | , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
07.12.2017
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Subjects | |
Online Access | Get full text |
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Summary: | Eugenol is a phenolic compound and the main constituent of the essential oil of clove India. Although there are reports of some pharmacological effects of eugenol, this study is the first that proposes to evaluate the antifungal effects of this phenol against both
and
cells. The effect of eugenol against yeast cells was analyzed for drug susceptibility, alterations in cell diameter, capsule properties, amounts of ergosterol, oxidative burst, and thermodynamics data. Data demonstrated that there is no interaction between eugenol and fluconazole and amphotericin B. Eugenol reduced the cell diameter and the capsule size, increased cell surface/volume, changed positively the cell surface charge of cryptococcal cells. We also verified increased levels of reactive oxygen species without activation of antioxidant enzymes, leading to increased lipid peroxidation, mitochondrial membrane depolarization and reduction of lysosomal integrity in cryptococcal cells. Additionally, the results showed that there is no significant molecular interaction between eugenol and
. Morphological alterations, changes of cellular superficial charges and oxidative stress play an important role in antifungal activity of eugenol against
and
that could be used as an auxiliary treatment to cutaneous cryptococcosis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Gordon Ramage, University of Glasgow, United Kingdom Reviewed by: Fernanda Lopes Fonseca, Centro de Desenvolvimento Tecnológico em Sáude (CDTS)/Fundação Oswaldo Cruz (Fiocruz), Brazil; Ryan Kean, University of the West of Scotland, United Kingdom This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2017.02419 |