Generation of induced pluripotent stem cells MMCi001-A from a Taiwanese hearing loss patient carrying GJB2 pV37I mutation

Hearing loss is the most common disorder in the sensory system. Mutations in GJB2 have been reported to be very common in sensorineural hearing loss patients. In this report, we generated an induced pluripotent stem cell (iPSC) line, MMCi001-A, from the peripheral blood mononuclear cells of a 4-year...

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Published inStem cell research Vol. 42; p. 101692
Main Authors Lu, Huai-En, Tsai, Chia-Ling, Chiu, Ing-Ming, Pan, Yu-Ling, Lin, Yi-Feng, Lin, Hung-Ching, Hsu, Yi-Chao
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.01.2020
Elsevier
Subjects
Animals
Cell Line
Child, Preschool
Connexins - metabolism
Hearing Loss - genetics
Humans
Induced Pluripotent Stem Cells - metabolism
Male
Taiwan
Taiwan
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Summary:Hearing loss is the most common disorder in the sensory system. Mutations in GJB2 have been reported to be very common in sensorineural hearing loss patients. In this report, we generated an induced pluripotent stem cell (iPSC) line, MMCi001-A, from the peripheral blood mononuclear cells of a 4-year-old male hearing loss patient carrying GJB2 pV37I mutation by using the Sendai virus delivery system. The generated iPSCs were demonstrated to express pluripotent markers and be differentiated into three germ layers in vitro and in vivo. This GJB2-pV37I iPSCs is valuable for studying the pathogenic mechanisms and drug discovery of hearing loss.
Bibliography:ObjectType-Case Study-2
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2019.101692