Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles
Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global tempe...
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Published in | Frontiers in microbiology Vol. 9; p. 2378 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
09.10.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global temperature rises. Here we demonstrate the use of a lytic enyzme, PeiR, from a methanogen virus that infects
M1 as an effective agent inhibiting a range of rumen methanogen strains in pure culture. We determined the substrate specificity of soluble PeiR and demonstrated that the enzyme is capable of hydrolysing the pseudomurein cell walls of methanogens. Subsequently,
was fused to the polyhydroxyalkanoate (PHA) synthase gene
and displayed on the surface of PHA bionanoparticles (BNPs) expressed in
one-step biosynthesis. These tailored BNPs were capable of lysing not only the original methanogen host strain, but a wide range of other rumen methanogen strains
Methane production was reduced by up to 97% for 5 days post-inoculation in the
assay. We propose that tailored BNPs carrying anti-methanogen enzymes represent a new class of methane inhibitors. Tailored BNPs can be rapidly developed and may be able to modulate the methanogen community
with the aim to lower ruminant methane emissions without impacting animal productivity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Donald Jay Ferguson, Miami University, United States; Antti Ilmari Vasala, BioSilta Oy, Finland This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology Edited by: Peter Neubauer, Technische Universität Berlin, Germany |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2018.02378 |