Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin
Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus , are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindere...
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| Published in | Frontiers in microbiology Vol. 10; p. 2675 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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22.11.2019
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| Abstract | Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus
, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of
strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a
strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of
described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. |
|---|---|
| AbstractList | Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus
, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of
strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a
strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of
described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin.Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus , are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. |
| Author | Chávez, Renato Díaz, Anaí Fierro, Francisco Villanueva, Pablo Oliva, Vicente Gil-Durán, Carlos Vaca, Inmaculada |
| AuthorAffiliation | 1 Departamento de Química, Facultad de Ciencias, Universidad de Chile , Santiago , Chile 2 Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile , Santiago , Chile 3 Departamento de Biotecnología, Universidad Autónoma Metropolitana, Unidad Iztapalapa , Mexico City , Mexico |
| AuthorAffiliation_xml | – name: 3 Departamento de Biotecnología, Universidad Autónoma Metropolitana, Unidad Iztapalapa , Mexico City , Mexico – name: 1 Departamento de Química, Facultad de Ciencias, Universidad de Chile , Santiago , Chile – name: 2 Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile , Santiago , Chile |
| Author_xml | – sequence: 1 givenname: Anaí surname: Díaz fullname: Díaz, Anaí – sequence: 2 givenname: Pablo surname: Villanueva fullname: Villanueva, Pablo – sequence: 3 givenname: Vicente surname: Oliva fullname: Oliva, Vicente – sequence: 4 givenname: Carlos surname: Gil-Durán fullname: Gil-Durán, Carlos – sequence: 5 givenname: Francisco surname: Fierro fullname: Fierro, Francisco – sequence: 6 givenname: Renato surname: Chávez fullname: Chávez, Renato – sequence: 7 givenname: Inmaculada surname: Vaca fullname: Vaca, Inmaculada |
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| Copyright | Copyright © 2019 Díaz, Villanueva, Oliva, Gil-Durán, Fierro, Chávez and Vaca. Copyright © 2019 Díaz, Villanueva, Oliva, Gil-Durán, Fierro, Chávez and Vaca. 2019 Díaz, Villanueva, Oliva, Gil-Durán, Fierro, Chávez and Vaca |
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| Keywords | Antarctica electroporation protoplasts transformation Pseudogymnoascus |
| Language | English |
| License | Copyright © 2019 Díaz, Villanueva, Oliva, Gil-Durán, Fierro, Chávez and Vaca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology Edited by: Stefanie Pöggeler, University of Göttingen, Germany Reviewed by: Skander Elleuche, Miltenyi Biotec, Germany; Daniela Nordzieke, University of Göttingen, Germany |
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| Title | Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
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