Analysis of loss of heterozygosity in neoplastic nodules induced by diethylnitrosamine in the resistant BFF1 rat strain

• Published in: Carcinogenesis (New York) Vol. 20; no. 7; pp. 1363 - 1368
• Main Authors: Gariboldi, Manuela, Pascale, Rosa, Manenti, Giacomo, Miglio, Maria R.De, Calvisi, Diego, Carru, Angelo, Dragani, Tommaso A., Feo, Francesco
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.07.1999; Oxford Publishing Limited (England)
• Subjects: (BN×F344)F1 hybrid; 2-Acetylaminofluorene; Animals; BFF1; Biological and medical sciences; Brown Norway; Carcinogenesis, carcinogens and anticarcinogens; Carcinoma, Hepatocellular - chemically induced; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; Chemical agents; Chromosome Mapping; Diethylnitrosamine; F344; Fisher 344; Genetic Markers; GST-P; HCC; hepatocellular carcinoma; Liver - drug effects; Liver - pathology; Liver Neoplasms - chemically induced; Liver Neoplasms - genetics; Liver Neoplasms - pathology; LOH; loss of heterozygosity; Loss of Heterozygosity - genetics; Male; Medical sciences; Microsatellite Repeats - genetics; placental glutathione S-transferase; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Rats; Rats, Inbred BN; Rats, Inbred F344; single sequence length polymorphism; SSLP; Tumors; Rat; Liver; Tumoral tissue; Rodentia; Hepatic disease; Malignant tumor; Resistance; Carcinogen; Vertebrata; Mammalia; Animal; Microsatellite DNA; Loss of heterozygosity; Digestive diseases; Genetics; Locus
• ISSN: 0143-3334; 1460-2180; 1460-2180
• DOI: 10.1093/carcin/20.7.1363

Loss of heterozygosity (LOH) at specific chromosomal regions is a frequent event in poorly differentiated human hepatocellular carcinomas (HCCs), but rare in mouse HCCs. This behavior could depend on interspecies differences in mechanisms of hepatocarcinogenesis or in developmental stage of lesions. To verify if LOH is involved in rat hepatocarcinogenesis, we studied LOH frequency in slowly growing neoplastic nodules induced by Solt–Farber model in diethylnitrosamine-initiated BFF1 rats. We analyzed, with microsatellites, markers at 67 rat loci dispersed over all chromosomes, corresponding to regions homologous to those lost in human HCCs or containing hepatocellular susceptibility (Hcs) or resistance (Hcr) loci in rat and mouse. In agreement with previous findings with mouse HCCs, but at variance with human HCCs, no detectable LOH was found at any locus in rats, suggesting rare LOH involvement in neoplastic nodules, with low tendency to progress to full malignancy, of BFF1 rats.