Staphylococcus aureus Colonization Is Increased on Lupus Skin Lesions and Is Promoted by IFN-Mediated Barrier Disruption

• Published in: Journal of investigative dermatology Vol. 140; no. 5; pp. 1066 - 1074.e4
• Main Authors: Sirobhushanam, Sirisha, Parsa, Navya, Reed, Tamra J., Berthier, Celine C., Sarkar, Mrinal K., Hile, Grace A., Tsoi, Lam C., Banfield, Josh, Dobry, Craig, Horswill, Alexander R., Gudjonsson, Johann E., Kahlenberg, J. Michelle
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2020
• Subjects: Adult; Cell Adhesion; Cell Movement; Cells, Cultured; Female; Filaggrin Proteins; Humans; Interferon Type I - metabolism; Interferon-gamma - metabolism; Intermediate Filament Proteins - genetics; Intermediate Filament Proteins - metabolism; Keratinocytes - physiology; Lupus Erythematosus, Systemic - immunology; Lupus Erythematosus, Systemic - microbiology; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Microarray Analysis; Middle Aged; Pregnancy; Sequence Analysis, RNA; Skin - immunology; Skin - microbiology; Skin - pathology; Staphylococcal Infections - immunology; Staphylococcal Infections - microbiology; Staphylococcus aureus - physiology; AD; CFU; HC; CLASI; SLE
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1016/j.jid.2019.11.016

Cutaneous inflammation is recurrent in systemic lupus erythematosus (SLE), yet mechanisms that drive cutaneous inflammation in SLE are not well defined. Type I IFNs are elevated in nonlesional SLE skin and promote inflammatory responses. Staphylococcus aureus, known to induce IFN production, could play a role in cutaneous inflammation in SLE. We show here that active cutaneous lupus erythematosus lesions are highly colonized (∼50%) by S. aureus. To define the impact of IFNs on S. aureus colonization, we examined the effects of type I and type II IFNs on S. aureus adherence and invasion. An increase in adherent S. aureus was observed after exposure to both IFN-α and -γ, whereas IFN-γ appeared to inhibit invasion of S. aureus. Cutaneous lupus erythematosus lesional skin microarray data and RNA sequencing data from SLE keratinocytes identified repression of barrier gene expression, such as filaggrin and loricrin, and SLE keratinocytes exhibited increased S. aureus–binding integrins. These SLE-associated changes could be replicated by IFN treatment of keratinocytes. Further, SLE keratinocytes exhibited increased binding to S. aureus. Together, these data suggest that chronic exposure to IFNs induces barrier disruption that allows for higher S. aureus colonization in SLE skin.