Quantitative Proteomic and Transcriptomic Analyses of Metabolic Regulation of Adult Reproductive Diapause in Drosophila suzukii (Diptera: Drosophilidae) Females

Diapause is a form of dormancy used by many insects to survive adverse environmental conditions, which can occur in specific developmental stages in different species. is a serious economic pest and we determined the conditions for adult reproductive diapause by the females in our previous studies....

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Published inFrontiers in physiology Vol. 10; p. 344
Main Authors Zhai, Yifan, Dong, Xiaolin, Gao, Huanhuan, Chen, Hao, Yang, Puyun, Li, Ping, Yin, Zhenjuan, Zheng, Li, Yu, Yi
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 04.04.2019
Subjects
Drosophila suzukii
Physiology
qRT-PCR
quantitative proteomics
reproductive diapause
RNA-Seq transcriptomics
reproductive diapause
Drosophila suzukii
quantitative proteomics
RNA-Seq transcriptomics
qRT-PCR
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Summary:Diapause is a form of dormancy used by many insects to survive adverse environmental conditions, which can occur in specific developmental stages in different species. is a serious economic pest and we determined the conditions for adult reproductive diapause by the females in our previous studies. In this study, we combined RNA-Seq transcriptomic and quantitative proteomic analyses to identify adult reproductive diapause-related genes and proteins. According to the transcriptomic analysis, among 242 annotated differentially expressed genes in non-diapause and diapause females, 129 and 113 genes were up- and down-regulated, respectively. In addition, among the 2,375 proteins quantified, 39 and 23 proteins were up- and down-regulated, respectively. The gene expression patterns in diapause- and non-diapause were confirmed by qRT-PCR or western blot analysis. The overall analysis of robustly regulated genes at the protein and mRNA levels found four genes that overlapped in the up-regulated group and six genes in the down-regulated group, and thus these proteins/genes may regulate adult reproductive diapause. These differentially expressed proteins/genes act in the citrate cycle, insulin signaling pathway, PI3K-Akt signaling pathway, and amino acid biosynthesis pathways. These results provide the basis for further studies of the molecular regulation of reproductive diapause in this species.
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This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology
Edited by: Youjun Zhang, Institute of Vegetables and Flowers (CAAS), China
Reviewed by: Gabriella Mazzotta, University of Padova, Italy; Shi Wangpeng, China Agricultural University, China
ISSN:1664-042X
1664-042X
DOI:10.3389/fphys.2019.00344