Preferential partitioning of melittin into the air/water interface: structural and thermodynamic implications

• Published in: Biophysical journal Vol. 71; no. 3; pp. 1422 - 1427
• Main Authors: Wackerbauer, G., Weis, I., Schwarz, G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.1996
• Subjects: Air; Animals; Biophysical Phenomena; Biophysics; Hydrogen-Ion Concentration; In Vitro Techniques; Melitten - chemistry; Membrane Potentials; Membrane Proteins - chemistry; Molecular Structure; Surface Properties; Thermodynamics; Water
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(96)79343-8

The membrane active agent melittin has been investigated with regard to the formation of a Langmuir monolayer and the accordingly induced surface activities. We show that in spite of its considerable solubility in an aqueous medium, this peptide nevertheless largely accumulates in the air/water interface unless the lateral pressure is raised beyond a certain threshold value depending on the pH in the subphase. The true surface concentrations have been determined by means of a recently developed novel method based on thermodynamic principles. It affords an access to the partitioning equilibrium between the surface and subphase domains, provided the latter surrounding is not excessively preferred. In the present case this approach was used to derive quantitative information on the pertinent interfacial structure and thermodynamics. In particular, the apparent molecular area and the Gibbs energy of mutual interaction in the monolayer could be evaluated as a function of the applied surface pressure. The data suggest the existence of two structural conversions in the course of an increasing lateral compression. The surface-associated peptide accordingly assumes three different states of successively reduced area requirements, supposedly owing to an orientational transition involving a straightening up of a helical conformation. This conclusion is corroborated by surface potential measurements reflecting corresponding changes of the effective dipole moment perpendicular to the surface.