New Insights into Clostridium difficile (CD) Infection in Latin America: Novel Description of Toxigenic Profiles of Diarrhea-Associated to CD in Bogotá, Colombia
(CD) produces antibiotic associated diarrhea and leads to a broad range of diseases. The source of CD infection (CDI) acquisition and toxigenic profile are factors determining the impact of CD. This study aimed at detecting healthcare facility onset- (HCFO) and community-onset (CO) CDI and describin...
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Published in | Frontiers in microbiology Vol. 9; p. 74 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
30.01.2018
|
Subjects | |
Online Access | Get full text |
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Summary: | (CD) produces antibiotic associated diarrhea and leads to a broad range of diseases. The source of CD infection (CDI) acquisition and toxigenic profile are factors determining the impact of CD. This study aimed at detecting healthcare facility onset- (HCFO) and community-onset (CO) CDI and describing their toxigenic profiles in Bogotá, Colombia. A total of 217 fecal samples from patients suffering diarrhea were simultaneously submitted to two CDI detection strategies: (i)
culture using selective chromogenic medium (SCM; chromID, bioMérieux), followed verification by colony screening (VCS), and (ii) molecular detection targeting constitutive genes, using two conventional PCR tests (conv.PCR) (conv.
y conv.
) and a quantitative test (qPCR.
). The CD toxigenic profile identified by any molecular test was described using 6 tests independently for describing
and
organization. High overall CDI frequencies were found by both SCM (52.1%) and conv.PCR (45.6% for conv.16S and 42.4% for conv.
), compared to reductions of up to half the frequency by VCS (27.2%) or qPCR.
(22.6%). Infection frequencies were higher for SCM and conv.
regarding HCFO but greater for CO concerning conv.
, such differences being statistically significant. Heterogeneous toxigenic profiles were found, including amplification with lok1/3 primers simultaneously with other
markers (
or
). These findings correspond the first report regarding the differential detection of CDI using
culture and molecular detection tests in Colombia, the circulation of CD having heterogeneous toxigenic profiles and molecular arrays which could affect the impact of CDI epidemiology. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Philip Arthur Mackowiak, University of Maryland, Baltimore, United States This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology Reviewed by: Venkataramana Mudili, Defence Research and Development Organisation – Bharathiar University, India; Paul Edward Carlson, Food and Drug Administration, United States |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2018.00074 |