Screening of wheat endophytes as biological control agents against Fusarium head blight using two different in vitro tests

• Published in: Microbiological research Vol. 202; pp. 11 - 20
• Main Authors: Comby, Morgane, Gacoin, Marie, Robineau, Mathilde, Rabenoelina, Fanja, Ptas, Sébastien, Dupont, Joëlle, Profizi, Camille, Baillieul, Fabienne
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2017; Elsevier
• Subjects: Antibiosis; Aureobasidium proteae; Bacteria - classification; Bacteria - growth & development; Bacteria - isolation & purification; Bacteria - metabolism; Coculture Techniques; Detached spikelets; Dual-culture; Endophytes - classification; Endophytes - growth & development; Endophytes - isolation & purification; Endophytes - metabolism; France; Fungi - classification; Fungi - growth & development; Fungi - isolation & purification; Fungi - metabolism; Fusarium - growth & development; Life Sciences; Phoma glomerata; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant Roots - microbiology; Sarocladium kiliense; Soil Microbiology; Triticum - microbiology; Aureobasidium proteae; Dual-culture; Detached spikelets; Phoma glomerata; Sarocladium kiliense
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2017.04.014

In order to find biological control agents (BCAs) for the management of Fusarium head blight (FHB), a major disease on wheat crops worldwide, 86 microorganisms isolated from inner tissues of wheat plants were discriminated for their ability to inhibit the growth of Fusarium graminearum and Fusarium culmorum by in vitro dual culture assays. A group of 22 strains appeared very effective to inhibit F. graminearum (inhibition of 30–51%) and they were also globally effective in controlling F. culmorum (inhibition of 15–53%). Further evaluation of a subselection of strains by screening on detached spikelets in vitro confirmed three species, namely Phoma glomerata, Aureobasidium proteae and Sarocladium kiliense, that have not yet been reported for their efficacy against Fusarium spp., indicating that looking for BCAs toward FHB among wheat endophytes proved to be promising. The efficacy of some strains turned out different between both in vitro screening approaches, raising the importance of finding the most appropriate screening approach for the search of BCAs. This study pointed out the interest of the test on detached wheat spikelets that provided information about a potential pathogenicity, the growth capacity and efficacy of the endophyte strains on the targeted plant, before testing them on whole plants.