Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus)

• Published in: Carbohydrate polymers Vol. 88; no. 2; pp. 522 - 529
• Main Authors: Tian, Yuting, Zeng, Hongliang, Xu, Zhenbo, Zheng, Baodong, Lin, Yuexu, Gan, Chunji, Lo, Y. Martin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 02.04.2012; Elsevier
• Subjects: Agaricus bisporus; Antioxidant activity; Antioxidants; Applied sciences; Basidiocarps; Biological and medical sciences; Carbohydrates; Exact sciences and technology; Extraction; General and cellular metabolism. Vitamins; Medical sciences; Molecular weight; mushrooms; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharide; Polysaccharides; raw materials; Regression analysis; response surface methodology; spectral analysis; Starch and polysaccharides; temperature; Temperature effects; Ultrasonics; Water temperature; Agaricus bisporus; Extraction; Polysaccharide; Antioxidant activity; DPPH; Molecular structure; Chemical isolation; Response surface methodology; Antioxidant; Experimental study; Solvent extraction; In vitro; Optimization; Basidiomycota; Operating conditions; Fungi; Radical trapping; Scavenging efficiency; Activity concentration relation; Plant origin; Hydroxyl radicals; Oside polymer; Ultrasound assisted process; Edible fungi
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2011.12.042

Ultrasonic-assisted extraction (UAE) of polysaccharides from white button mushroom (Agaricus bisporus) was studied. The four parameters, ultrasonic power, extraction temperature, extraction time, and the ratio of water volume to raw material weight (W/M ratio), were optimized using the central composite design (CCD) with a quadratic regression model built by using response surface methodology (RSM). The optimal extraction conditions found were: ultrasonic power 230W, extraction temperature 70°C, extraction time 62min, and W/M ratio 30ml/g, where the highest A. bisporus polysaccharides (ABPS) yield of 6.02% was achieved. From HPGPC analysis, the molecular weight of ABPS was 158kDa. Chemical and spectroscopic studies illustrated ABPS was only composed of Glc with β-type glycosidic bond. In addition, the antioxidative activity of ABPS was investigated by measuring its scavenging ability on DPPH and hydroxyl in vitro. The results indicated that ABPS has good antioxidant activity.