Peripheral Monocytes of Obese Women Display Increased Chemokine Receptor Expression and Migration Capacity

• Published in: The journal of clinical endocrinology and metabolism Vol. 99; no. 7; pp. 2500 - 2509
• Main Authors: Krinninger, P., Ensenauer, R., Ehlers, K., Rauh, K., Stoll, J., Krauss-Etschmann, S., Hauner, H., Laumen, H.
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.07.2014; Copyright by The Endocrine Society
• Subjects: Adipose tissue; Adult; Case-Control Studies; CC chemokine receptors; CD14 antigen; CD16 antigen; Cell activation; Chemokine receptors; Chemokines; Chemotactic response; Chemotaxis, Leukocyte - genetics; CXCR3 protein; Diabetes mellitus (non-insulin dependent); Female; Flow cytometry; Gene Expression; Humans; Ideal Body Weight; Insulin resistance; Leukocyte migration; Leukocytes (mononuclear); Monocyte chemoattractant protein 1; Monocytes; Monocytes - metabolism; Obesity; Obesity - blood; Obesity - genetics; Peripheral blood mononuclear cells; Receptors, Chemokine - genetics; Receptors, Chemokine - metabolism; Thinness - blood; Thinness - genetics; Up-Regulation - genetics
• ISSN: 0021-972X; 1945-7197; 1945-7197
• DOI: 10.1210/jc.2013-2611

Context:The activation of peripheral immune cells and the infiltration of immune cells into adipose tissue in obesity are implicated in the development of type 2 diabetes mellitus.Objective:The aim of the study was to compare peripheral immune cells from obese and normal-weight women with regard to composition of immune cell subpopulations, surface expression of the chemokine receptors (CCRs) CCR2, CCR3, CCR5, and CXCR3 (chemokine (C-X-C motif) receptor 3) and cell-intrinsic migration capacity.Design:This was a case-control study.Setting:The study was conducted at a university clinical study center.Patients:Obese females and normal-weight females were included for fluorescence-activated cell sorting analysis and migration assays.Main Outcome Measures:Peripheral blood mononuclear cells were prepared from fasting blood samples and used for fluorescence-activated cell sorting analysis and migration assays.Results:An increase in the percentages of CD14+CD16+ monocytes was observed in obese subjects compared with controls. The CCR profile of monocytes differed significantly in the obese state; in particular, CCR2 levels were increased. In addition, a higher chemotactic activity of monocytes from obese subjects was observed in a migration assay, which was associated with both insulin resistance and CCR2 expression.Conclusion:Our results suggest that the enhanced intrinsic migratory capacity of peripheral monocytes in obese women may be due to the increased CCR expression, further supporting a link between peripheral immune cell dysfunction and obesity.