Preparation and characterization of trace elements-multidoped injectable biomimetic materials for minimally invasive treatment of osteoporotic bone trauma

• Published in: Journal of biomedical materials research. Part A Vol. 95A; no. 4; pp. 1170 - 1181
• Main Authors: Yang, Xianyan, Gan, Yilai, Gao, Xin, Zhao, Li, Gao, Changyou, Zhang, Xinli, Feng, Yanbo, Ting, Kang, Gou, Zhongru
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.12.2010; Wiley-Blackwell
• Subjects: Animals; Biological and medical sciences; Biomimetic Materials - chemical synthesis; Biomimetic Materials - pharmacology; Biomimetic Materials - therapeutic use; Bone Density - drug effects; Calcium Phosphates - pharmacology; Ceramics - pharmacology; Diseases of the osteoarticular system; Female; Femur - diagnostic imaging; Femur - drug effects; Femur - pathology; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; Hydrogen-Ion Concentration - drug effects; injectable biomaterials; Injections; Injuries of the limb. Injuries of the spine; Ions; Materials Testing - methods; Medical sciences; Microscopy, Electron, Scanning; minimally invasive therapy; multidoping; Osteoporosis - diagnostic imaging; Osteoporosis - drug therapy; Osteoporosis - pathology; Osteoporosis - physiopathology; Osteoporosis. Osteomalacia. Paget disease; osteoporotic trauma; Rats; Rats, Sprague-Dawley; Rheology - drug effects; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; trace elements; Trace Elements - pharmacology; Trace Elements - therapeutic use; Traumas. Diseases due to physical agents; Water - chemistry; X-Ray Microtomography; Injectable form; Diseases of the osteoarticular system; osteoporotic trauma; Trauma; minimally invasive therapy; multidoping; Osteoarticular system; Trace element; Osteoporosis; Treatment; trace elements; Preparation; injectable biomaterials; Biomaterial; Bone; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.32936

It has always been a clinical challenge to use the proper implants or biomaterials to restore function to traumatized bone defects in patients with osteoporosis. In this study, we prepared the alginate–chitosan/trace elements‐multidoped octacalcium phosphate–bioglass (AT–CS/teOCP‐BG) hydrogel composite as an injectable biomaterial and evaluated its physicochemical properties and biological performance. Trace amount of silicon, strontium, and zinc was first doped into the structure of OCP porous microspheres via a wet chemical reaction. The AT–CS complex was mechanically mixed with teOCP and then neutralized by the ionic products of bioglass 58S particles dissolution. Formulations of this novel composite presented in wet state have teOCP‐BG contents ranging from 7.8 to 25.4% and AT–CS content of below 3.2%. The composite retained gel in culture medium at 37°C, and the ratio of storage modulus and loss modulus (G′/G″) exhibited a marked increase with decreasing the amount of BG, signifying a pH‐dependent enhancement of rheological properties and gel stability. After injection into rat femoral bone marrow cavity with minimal tissue invasion, new bone ingrowth was observed from radiologic images in the ovariectomized (OVXed) rats, which was significantly greater than that found in sham‐operated animals within 8 weeks postoperatively. The fast bone regeneration phenomenon was observed in the OVXed rats by radiographic and microcomputerized tomography examination and histological analysis. These findings suggest that the AT–CS/teOCP‐BG system might be used as an injectable biomaterial for minimally invasive treatment of osteoporosis‐related (micro‐)trauma. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.