RAPD typing of clinical isolates of Staphylococcus haemolyticus

The randomly amplified polymorphic DNA (RAPD) assay was used to generate DNA fingerprints from clinical isolates of Staphylococcus haemolyticus isolated from patients treated with continuous ambulatory peritoneal dialysis and previously subjected to a combination of typing methods. The RAPD profiles...

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Bibliographic Details
Published inLetters in applied microbiology Vol. 18; no. 2; p. 86
Main Authors Young, K A, Power, E G M, Dryden, M S, Phillips, I
Format Journal Article
LanguageEnglish
Published England 01.02.1994
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Summary:The randomly amplified polymorphic DNA (RAPD) assay was used to generate DNA fingerprints from clinical isolates of Staphylococcus haemolyticus isolated from patients treated with continuous ambulatory peritoneal dialysis and previously subjected to a combination of typing methods. The RAPD profiles generated with one of six randomly designed 10-mer primers allowed visual discrimination of strains. Good correlation with the original typing scheme was achieved but RAPD typing allowed discrimination of strains previously indistinguishable.
ISSN:1472-765X
DOI:10.1111/j.1472-765X.1994.tb00811.x