Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation

• Published in: Biotechnology progress Vol. 29; no. 5; pp. 1116 - 1123
• Main Authors: Satomura, Atsushi, Katsuyama, Yoshiaki, Miura, Natsuko, Kuroda, Kouichi, Tomio, Ayako, Bamba, Takeshi, Fukusaki, Eiichiro, Ueda, Mitsuyoshi
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.09.2013
• Subjects: Adaptation, Physiological - genetics; breeding; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation, Fungal; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Hot Temperature; Metabolomics; Oligonucleotide Array Sequence Analysis; RNA, Fungal - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; thermotolerant yeast; trehalose; Trehalose - metabolism; trehalose; Saccharomyces cerevisiae; thermotolerant yeast; breeding
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1002/btpr.1754

A thermotolerant Saccharomyces cerevisiae yeast strain, YK60‐1, was bred from a parental strain, MT8‐1, via stepwise adaptation. YK60‐1 grew at 40°C, a temperature at which MT8‐1 could not grow at all. YK60‐1 exhibited faster growth than MT8‐1 at 30°C. To investigate the mechanisms how MT8‐1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress‐responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60‐1. Furthermore, nontargeting metabolome analysis showed that YK60‐1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8‐1. In conclusion, S. cerevisiae MT8‐1 acquired thermotolerance by induction of specific stress‐responsive genes and enhanced intracellular trehalose levels. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1116–1123, 2013